The experiments reported in the present dissertation were undertaken to obtain further evidence for the possible roles of protein, RNA and DNA macromolecules in radiation-mutagenesis in Drosophila melanogaster. Several antibiotics were tested for their modifying effects on the frequency of radiation-induced sex-linked recessive lethals.
Pre-radiation treatment with actinomycin D significantly reduces the frequency of induced mutations in germ cell stages assumed to include spermatids and spermatocytes. These results are consistent with the hypothesis of a role of proteins in the stabilization (repair) of radiation-induced premutational lesions.
Puromycin, a specific inhibitor of protein synthesis is ineffective in the modification of induced mutation frequencies in Drosophila melanogaster.
Mitomycin C, is itself a potent mutagen in all germ cell stages, peak mutagenicity occurring in spermatid stages. In combination with ɣ-rays, mitomycin C shows an overall additivity of effect. Mutation frequencies due to mitomycin C are not altered by pre- or concurrent treatment with actinomycin D. This may indicate a different mechanism for mutagenesis by mitomycin C and radiation. / Science, Faculty of / Zoology, Department of / Graduate
| Identifer | oai:union.ndltd.org:UBC/oai:circle.library.ubc.ca:2429/37527 |
| Date | January 1965 |
| Creators | Mukherjee, Ramendra Nath |
| Publisher | University of British Columbia |
| Source Sets | University of British Columbia |
| Language | English |
| Detected Language | English |
| Type | Text, Thesis/Dissertation |
| Rights | For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. |
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