Rebamipide is a mucoprotective agent commonly used to prevent nonsteriodal anti-inflammatory drug-induced gastrointenstinal side effects [1]. Human plasma and urine analysis of rebamipide utilizing high performance liquid chromatography (HPLC) have been reported [2]. Recently, we reported on the plasma levels of rebamipide in presense or absence of celecoxib or diclofenac in rats [3] using a modified HPLC method of detection developed by Jeoung et al. [4]. To tailor the method towards use in urinary rebamipide extraction and analysis, the following modifications were made:To compensate for high concentrations of rebamipide found in urine, a new rebamipide stock solution was prepared with a final concentration of 50,000 ng/mL.Rat urine calibration standards were obtained within the range of 50-1000 ng/mL and 1000-50,000 ng/mL.Plasma samples were replaced with urine samples.
Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-17039 |
Date | 01 January 2014 |
Creators | Cooper, Dustin L., Harirforoosh, Sam |
Publisher | Digital Commons @ East Tennessee State University |
Source Sets | East Tennessee State University |
Detected Language | English |
Type | text |
Format | application/pdf |
Source | ETSU Faculty Works |
Rights | http://creativecommons.org/licenses/by/4.0/ |
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