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Molecular mechanisms of brain-ras hyperactivity upon fluid balance, and sufficiency of angiotensin production from the subfornical organ to affect fluid balance

Fluid balance is critical for cells to maintain at homeostasis as disturbances in it can disrupt cellular function and consequently the physiology of an organism. Fluid loss for an organism can be classified as either intra- or extracellular, and it appears that different mechanisms have developed to restore homeostasis after intra- or extracellular dehydration. The renin-angiotensin system (RAS) has been shown to be an important mediator of extracellular dehydration induced fluid intake. Various lines of evidence have demonstrated the importance of the subfornical organ (SFO) to mediate fluid intake, especially due to the RAS, and we have shown that production and action of angiotensin (ANG) at the SFO is necessary for fluid intake due to ANG within the brain. Protein kinase C (PKC), specifically PKC-a;, is shown to be a necessary and sufficienty sufficient effector in the SFO to mediate brain angiotensin-II (ANG-II) polydipsia. It is also demonstrated that production of ANG from the SFO is sufficient to increase fluid intake through the ANG-II type 1 (AT1R) receptor and PKC. While production of ANG from the SFO is sufficient to increase fluid intake it is not sufficient to increase blood pressure, metabolism, or sodium appetite. Thus, production and action of ANG to activate PKC-a; is both necessary and sufficient to increase fluid intake at the SFO, and the fluid, pressor, and metabolic phenotypes of brain ANG through the SFO can be separated.

Identiferoai:union.ndltd.org:uiowa.edu/oai:ir.uiowa.edu:etd-5622
Date01 May 2015
CreatorsCoble, Jeffrey
ContributorsSigmund, Curt Daniel
PublisherUniversity of Iowa
Source SetsUniversity of Iowa
LanguageEnglish
Detected LanguageEnglish
Typedissertation
Formatapplication/pdf
SourceTheses and Dissertations
RightsCopyright © 2015 Jeffrey Coble

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