The purpose of this study is to explore the biological effects of ultrasound exposure on growth of Saccharomyces cerevisiae. The result shows the biological effects of ultrasound exposure. The resonance frequencies and a non-resonance frequency were used with different intensities to find the relations between ultrasonic and cell growth. The methods of this study adopted finite element method and Rayleigh-Plesset theory to calculate the resonance frequencies. And then, the study set a diffuse field to exposure the yeast cells. In the experiment, cell growths were analyzed by a 600 nm ultraviolet spectrophotometer measuring the cell mass concentration.
The results show that the restrained time under high intensity ultrasonic exposure was longer than low intensity in 0.306 and 11.6625 MHz. However, the restrained effects were the same between high and low irradiation intensity in 2.4079 MHz. By 10 MHz low irradiation intensity, the restrained time was 0.5 hr after sonication. The restrain effects were not obvious under low and high irradiation intensity in 16.124 MHz.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0208109-165113 |
| Date | 08 February 2009 |
| Creators | Zhang, Yong-cheng |
| Contributors | Shiuh-kuang Yang, Shao-yi Hisa, Ching-mei Hsu, Yi-Cheng Huang, Syue-jian Wu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0208109-165113 |
| Rights | not_available, Copyright information available at source archive |
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