The problem of the long-term proliferation of cells is a seminal one. It has always been a hot subject in biology. In this reach, it try to improve the growth rate of paramecium by ultrasound exposure. To perform the above-mentioned research, the oscillation of the paramecium in response to the ultrasound radiation is simulated using Rayleigh-Plesset¡¦s bubble activation theory. The gas body activation theory is to calculate the resonant frequencies of the paramecium at different stages of its life.
In this research, it will study the activation of the paramecium division using the resonant and non-resonant frequency of the ultrasonic exposure. The observing images obtained from a microscopic camera can be made and recorded by a personal computer. The biological effects such as the hatching period, growth rate, etc., can be observed from the images. In the past, the physical measurement such as the counting method is the easiest way to observe the biological effects of ultrasound. However, it is not sufficiently to analyze the quantitative bioeffect of paramecium by taking counts. Therefore, utilizing the biochemical technique to assay the difference of specimen may be a good point of view. In this research, employing BCA (Bicinchoninic Acid) assay to inspect the protein variation attain a brand-new quantitative analysis. The results obtained from this research can be used to develop the related ultrasonic biotechnology.
By using the theory, the calculated resonance frequency of the paramecium vacuole is about 0.467~1.27 MHz. The maximum amount of paramecium was observed in 72th hours with 1 MHz ultrasound exposure for 5 minutes. It¡¦s 1.77 times relative to the control.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0210104-010649 |
| Date | 10 February 2004 |
| Creators | Chiu, Wen-Kuei |
| Contributors | none, S.Y. Hsia, S. K. Yang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0210104-010649 |
| Rights | not_available, Copyright information available at source archive |
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