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An investigation of some biochemical and cellular properties of subretnal fluids.

by Xu Xun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 78-87). / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / Chapter CHAPTER 2 --- LITERATURE REVIEW --- p.6 / Chapter 2.1 --- Anatomy of retina and vitreous --- p.6 / Chapter 2.2 --- Rhegmatogenous retinal detachment --- p.8 / Chapter 2.2.1 --- Retinal breaks --- p.8 / Chapter 2.2.2 --- Retinal detachment and subretinal fluid --- p.8 / Chapter 2.3 --- Proliferative vitreoretinopathy --- p.9 / Chapter 2.4 --- Total protein in subretinal fluid --- p.11 / Chapter 2.5 --- Fibroblast growth factor --- p.12 / Chapter 2.5.1 --- Structure of b and a FGF and their gene --- p.13 / Chapter 2.5.2 --- Expression of bFGF and aFGF in neuroretinal and pigment epithelial cells --- p.14 / Chapter 2.5.3 --- The FGF receptors --- p.15 / Chapter 2.5.4 --- In vitro biological effect of FGF --- p.15 / Chapter 2.5.5 --- FGF in retinal diseases --- p.16 / Chapter 2.6 --- Cellular study of proliferative vitreoretinopathy --- p.18 / Chapter 2.6.1 --- Experimental study --- p.18 / Chapter 2.6.2 --- Pathogenesis of intravitreal proliferation --- p.20 / Chapter 2.6.3 --- Cellular components of proliferative tissue --- p.20 / Chapter 2.6.4 --- Cellular components of subretinal fluid --- p.22 / Chapter CHAPTER 3 --- MATERIALS AND METHODS --- p.24 / Chapter 3.1 --- Specimens --- p.24 / Chapter 3.2 --- Determination of total protein --- p.24 / Chapter 3.2.1 --- Study population --- p.24 / Chapter 3.2.2 --- Quantitation of total protein --- p.25 / Chapter 3.3 --- Determination of bFGF --- p.27 / Chapter 3.3.1 --- Study population --- p.27 / Chapter 3.3.2 --- Quantitation of bFGF --- p.28 / Chapter 3.4 --- Celular study --- p.30 / Chapter 3.4.1 --- Study population --- p.30 / Chapter 3.4.2 --- Fixation of samples --- p.30 / Chapter 3.4.3 --- Immunocytology --- p.32 / Chapter 3.4.4 --- Examination of autofluorescence --- p.35 / Chapter 3.4.5 --- Hematoxylin and eosin staining --- p.36 / Chapter CHAPTER 4 --- RESULTS --- p.37 / Chapter 4.1 --- Total protein --- p.37 / Chapter 4.1.2 --- Total protein in subretinal fluids --- p.38 / Chapter 4.1.3 --- Total protein in normal vitreous of autopsy and sera of patients --- p.40 / Chapter 4.1.4 --- Relationship of protein level and size of retinal break --- p.40 / Chapter 4.1.5 --- Relationship of protein level and duration of retinal detachment --- p.43 / Chapter 4.1.6 --- Relationship of protein level and degree of PVR --- p.45 / Chapter 4.2 --- Basic FGF in subretinal fluids --- p.47 / Chapter 4.2.1 --- Standard curve for determination of bFGF in SRF --- p.47 / Chapter 4.2.2 --- The levels of bFGF in both SRF and controls --- p.48 / Chapter 4.2.3 --- Levels of bFGF in different degrees of PVR --- p.50 / Chapter 4.2.4 --- Levels of bFGF in SRF of RD with and without previous cryotherapy --- p.54 / Chapter 4.2.5 --- The relationship of level of protein and bFGF --- p.55 / Chapter 4.3 --- Results of cytological examination --- p.57 / Chapter 4.3.1 --- Pigment examingnation by autofluorescence --- p.57 / Chapter 4.3.2 --- Cellular study of subretinal fluids --- p.59 / Chapter 4.3.3 --- Cellular study of subretinal fluid in eyes with prior cryotherapy --- p.62 / Chapter CHAPTER 5 --- DISCUSSION --- p.68 / Chapter 5.1 --- Evaluaton of method for obtaining specimens --- p.68 / Chapter 5.2 --- Total protein of SRF in retinal detachment --- p.69 / Chapter 5.3 --- Basic FGF in subretinal fluids --- p.71 / Chapter 5.4 --- Elevated level of bFGF in eye after cryotherapy --- p.74 / Chapter 5.5 --- Cell components in SRF of PVR --- p.75 / REFERENCES --- p.78

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318156
Date January 1994
ContributorsXu, Xun., Chinese University of Hong Kong Graduate School. Division of Surgical Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, vi, 87 leaves : ill. (some mounted col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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