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Studies on ADP-Ribose Polymer Metabolism in Cultured Mammalian Cells Following DNA Damage

ADP-ribose polymer metabolism has been studied in human cells derived from a patient with Glutamyl Ribose Phosphate Storage Disease (GRPSD) and in mouse C3H1OT1/2 cells following oxidative stress induced by hydrogen peroxide (H202 ). It has been postulated that GRPSD resulted from an abnormality in ADP-ribose polymer metabolism. This study has shown that these cells exhibit reduced poly(ADP ribose) polymerase activity which is proposed to result from modification of the enzyme with ribose phosphate groups. The modification in the polymerase is proposed to be secondary to a defect in either ADP-ribosyl proteinlyase or an overproduction of a cellular phosphodiesterase. The metabolism of ADP-ribose polymers was rapidly altered by H202 and there were independent effects on adenine nucleotide pools. The results suggest that ADP-ribose polymer metabolism is involved in cellular defenses to oxidative stress.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc500354
Date05 1900
CreatorsMaharaj, Geeta
ContributorsJacobson, Myron, Lacko, Andras G., Harris, Ben G.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatx, 130 leaves: ill., Text
RightsPublic, Maharaj, Geeta, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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