Two different cloned human DNA segments encompassing transfer RNA gene and pseudogene clusters have been isolated from a human gene library harbored in bacteriophage lambda Charon 4-A. One clone (designated as λhVal7) encompassing a 20.5-kilobase (Kb) human DNA insert was found to contain a valine transfer RNA_AAC gene and several Alu-like elements by Southern blot hybridization analysis and DNA sequencing with the dideoxyribonucleotide chain-termination method in the bacteriophage M13mp19 vector. Another lambda clone (designated as λhLeu8) encompassing a 14.3-Kb segment of human DNA was found to contain a methionine elongator transfer RNA_CAT pseudogene and other as yet unidentified transfer RNA pseudogenes.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc500850 |
| Date | 12 1900 |
| Creators | Lee, Mike Ming-Jen |
| Contributors | Pirtle, Robert M., Norton, S. J., Wu, Edward Ming-chi, 1938- |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | viii, 100 leaves: ill., Text |
| Rights | Public, Lee, Mike Ming-Jen, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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