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N-of-1-pathways MixEnrich: advancing precision medicine via single-subject analysis in discovering dynamic changes of transcriptomes

Background: Transcriptome analytic tools are commonly used across patient cohorts to develop drugs and predict clinical outcomes. However, as precision medicine pursues more accurate and individualized treatment decisions, these methods are not designed to address single-patient transcriptome analyses. We previously developed and validated the N-of-1-pathways framework using two methods, Wilcoxon and Mahalanobis Distance (MD), for personal transcriptome analysis derived from a pair of samples of a single patient. Although, both methods uncover concordantly dysregulated pathways, they are not designed to detect dysregulated pathways with up- and down-regulated genes (bidirectional dysregulation) that are ubiquitous in biological systems. Results: We developed N-of-1-pathways MixEnrich, a mixture model followed by a gene set enrichment test, to uncover bidirectional and concordantly dysregulated pathways one patient at a time. We assess its accuracy in a comprehensive simulation study and in a RNA-Seq data analysis of head and neck squamous cell carcinomas (HNSCCs). In presence of bidirectionally dysregulated genes in the pathway or in presence of high background noise, MixEnrich substantially outperforms previous single-subject transcriptome analysis methods, both in the simulation study and the HNSCCs data analysis (ROC Curves; higher true positive rates; lower false positive rates). Bidirectional and concordant dysregulated pathways uncovered by MixEnrich in each patient largely overlapped with the quasi-gold standard compared to other single-subject and cohort-based transcriptome analyses. Conclusion: The greater performance of MixEnrich presents an advantage over previous methods to meet the promise of providing accurate personal transcriptome analysis to support precision medicine at point of care.

Identiferoai:union.ndltd.org:arizona.edu/oai:arizona.openrepository.com:10150/625841
Date24 May 2017
CreatorsLi, Qike, Schissler, A. Grant, Gardeux, Vincent, Achour, Ikbel, Kenost, Colleen, Berghout, Joanne, Li, Haiquan, Zhang, Hao Helen, Lussier, Yves A.
ContributorsUniv Arizona, Ctr Biomed Informat & Biostat, Univ Arizona, Inst Bio5, Univ Arizona, Dept Med, Univ Arizona, Grad Interdisciplinary Program Stat, Univ Arizona, Dept Math, Univ Arizona, Canc Ctr
PublisherBIOMED CENTRAL LTD
Source SetsUniversity of Arizona
LanguageEnglish
Detected LanguageEnglish
TypeArticle
Rights© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.
Relationhttp://bmcmedgenomics.biomedcentral.com/articles/10.1186/s12920-017-0263-4

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