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Previous issue date: 2017-04-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / As prote?nas da fam?lia triggering receptor expressed on myeloid cells (TREM) s?o
associados com o risco e progress?o da aterosclerose. Em resultados pr?vios observamos que
a alta express?o do TREML4est? relacionada com fase inicial da S?ndrome Coronariana
Aguda (SCA).Neste estudo, investigamos a rela??o dos polimorfismos e a express?o do
RNAm do TREML4 em leuc?citos de pacientes estratificados de acordo com a extens?o das
les?es das atr?rias coron?rias. Foram inclu?dos pacientes com doen?a arterial coronariana
(DAC) (n=151), com idades entre 30 e 74 anos, submetidos ? angiografia coron?ria eletiva. A
extens?o da les?o coronariana foi avaliada atrav?s do ?ndice de Friesinger. Os
polimorfismosrs2803495 (A> G), rs2803496 (T> C) e aexpress?o do RNAm do TREML4 em
leuc?citos foram analisadas por qRT-PCR. A express?o de TREML4 foi maior em pacientes
com les?es coronarianas graves em rela??o aos sem les?o, baixas e intermedi?rias, (p <0.05).
Indiv?duos portadores do alelo G do rs2803495 (gen?tipos AG / GG) e do alelo C do
rs2803496(gen?tipos TC / CC) apresentaram uma express?o de RNAm de TREML4 positiva
(Alelo G: OR = 2.4, 95% CI 1.0-5.7, p < 0.05; e Alelo C: OR = 7.8, 95% CI = 2.9-20.9, p <
0.01, respectivamente).Entretanto, os polimorfismos do TREML4 n?o foram associados com
as extens?es das les?es coron?rias. Em conclus?o, a express?o aumentada de mRNA de
TREML4 nos leuc?citos ? influenciada por polimorfismos e est? associada a les?es mais
graves das art?rias coron?rias, sugerindo seu papel como potencial biomarcador para
investigar a extens?o da les?o coronariana em pacientes com DAC. / The members of the triggering receptor expressed on myeloid cells (TREM) family are
associated with the risk and progression of atherosclerosis. We previously reported the
upregulation of TREML4 in the early phase of theacute coronary syndrome. Here, we
investigated the relationship of TREML4 polymorphisms and mRNA expression in blood
leukocytes of patients stratificate accordingto the extension of coronary artery lesion. Patients
with coronary artery disease (CAD) (n = 151), aged 30 to 74 years and undergoing elective
coronary angiography,were selected. The extension of coronary lesion was assessed by the
Friesinger index.TREML4 rs2803495 (A>G) and rs2803496 (T>C) variants andleukocyte
mRNA expression were analyzed by qRT-PCR. TREML4 expression was higher in patients
with major coronary artery lesions compared to subjects without or with low and intermediate
lesions, (p < 0.05). Subjects carrying rs2803495 G allele (AG/GG genotypes) and rs2803496
C allele (TC/CC genotypes) were more prone to have positive TREML4 mRNA expression
(Allele G: OR = 2.4, 95% CI 1.0-5.7, p < 0.05; allele C: OR = 7.8, and 95% CI = 2.9-20.9, p <
0.01, respectively).However, TREML4 polymorphisms were not associated with the extent of
coronary lesion.In conclusion, increased TREML4 mRNA expression in blood leukocytes is
influenced by gene polymorphisms and it is associated with more severe coronary artery
lesions, suggesting its role as potential biomarker to investigate the extent of coronary lesion
in CAD patients.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/23420 |
| Date | 26 April 2017 |
| Creators | Duarte, Victor Hugo Rezende |
| Contributors | 32160245801, http://lattes.cnpq.br/6121935907512568, Lajus, Tirzah Braz Petta, 03254085485, http://lattes.cnpq.br/9979644969955564, Caland, Lilia Basilio de, 89132173334, http://lattes.cnpq.br/4055624426698955, Luchessi, Andr? Ducati, Silbiger, Vivian Nogueira |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS FARMAC?UTICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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