Samples of different varieties grapevine from Małopolska and Podkarpacie collected on spring 2016 were tested for Grapevine Pinot gris virus (GPGV) by RT-PCR. 16 out of 65 plants were found positive for GPGV. Two set of primers were used for detection, targeting partial movement protein and coat protein, and RdRp domain, both of those regions were sequenced. Phylogenetic analyses settled the Polish isolates to described before asymptotic group. Also GPGV positive samples were tested by multiplex and simplex RT- PCR for multiple infections, and 18,5% of all samples were GPGV and GFkV positive. This was first survey of GPGV in Poland.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:424857 |
| Date | January 2017 |
| Creators | Pieczonka, Karolina Katarzyna |
| Source Sets | Czech ETDs |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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