Characteristics of methionine uptake by brush border membrane vesicles of bovine small intestine were investigated. Alkaline phosphatase marked the brush border membrane fraction obtained through differential centrifugation followed by a sucrose gradient. This preparation yielded a 10-fold enrichment of activity over homogenate. Methionine uptake was found to be into an osmotically active space. A binding constant of 75.4 pmol/mg membrane protein was determined. A significant (p<.05) sodium stimulation of methionine uptake was observed. This indicated active (energy-dependent) transport in addition to the diffusive component of intravesicular mention accumulation. Decreasing the pH of buffer medium significantly (p<.05) depresses methionine transport. A K<sub>m</sub> = .114 mM and V<sub>MAX</sub> = 56.5 pmol/s/mg membrane protein were ascertained for methionine. / M.S.
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/106167 |
| Date | January 1987 |
| Creators | Dahl, Geoffrey Eliot |
| Contributors | Dairy Science |
| Publisher | Virginia Polytechnic Institute and State University |
| Source Sets | Virginia Tech Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis, Text |
| Format | vi, 47 leaves, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 17368648 |
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