ABSTRACT
Bacteriophages have sparked interest as novel ways to control foodborne
pathogens. The application of Salmonella-specific phages as antimicrobial agents
was tested against relevant Salmonella isolates of poultry origin. Two different
Salmonella-specific phages, A and C, were isolated from enriched sewage. They
displayed differences in their host-range but exhibited virulent behaviour towards
Salmonella enterica serovar Typhimurium ATCC 13311. Toxicity studies were
conducted with individual and combined applications of phages A and C, at an
MOI of 1, on Salmonella ATCC 13311. Following 3 hour exposure, both
applications were equally effective at reducing Salmonella by approximately 1 x
105 CFU/ml. Similar toxicity profiles were observed with both applications,
however, a delay occurred with phage A. We propose that phages A and C have
similar infective specificities and that during combination competition for the
receptor is overcome by phage C. Neither application eliminated Salmonella to
undetectable levels. The presence of phage-resistant mutants is a fundamental
issue that will hamper the use of phages as alternate antimicrobial agents.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:wits/oai:wiredspace.wits.ac.za:10539/4674 |
| Date | 18 March 2008 |
| Creators | Hobbs, Angela |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 1548975 bytes, application/pdf, application/pdf |
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