This thesis is focused on the development and evaluation of different
hybrid scaffolds for the treatment of injuries in cartilage or bone.
These hybrid materials were three-dimensional polycaprolactone
macroporous scaffolds obtained through freeze extraction and particle
leaching combined method and modified with hyaluronic acid or
mineral particles. In order to facilitate the description of the obtained
results, the thesis is divided in two sections dedicated to bone and
cartilage tissue engineering respectively.
In the case of bone tissue engineering we addressed the treatment of
disorders associated with the spine that require spinal immobilization.
This Thesis proposes the development of a synthetic macroporous
support for intervertebral fusion as an alternative to commercial bone
substitutes. Macroporous scaffolds were developed with bare
polycaprolactone or its blends with polylactic acid in order to increase
its mechanical properties and degradation rate. Furthermore, the
scaffolds obtained were reinforced with hydroxyapatite or
Bioglass®45S5 to improve their mechanical properties and turn them
in bioactive scaffolds. The supports were characterized
physicochemically and biologically to determine if they met the
requirements of the project. Finally, materials were tested in vivo in a
bone critical size defect preformed in a rabbit model against a
commercial support.
Cartilage engineering has been extensively studied in the last years
due to the inherent limited self repair ability of this tissue. The second
part of the thesis was focused in developing a construct composed by in vitro differentiated chondrocyte like cells in a hybrid scaffold for
cartilage tissue engineering. Polycaprolactone hybrid substrates coated
with hyaluronic acid scaffold were developed obtaining a substrate
with positive influence over the development of chondrocyte
phenotype in culture and able to protect the cells from excessive
mechanical loading in the joint. Cell-scaffolds constructs were
obtained combining hybrid scaffolds with mesenchymal stem cells
and differentiating them to chondrocytes using chondrogenic culture
medium combined with hypoxia, mechanical stimulus or co-culture.
Finally the cellularized scaffolds were mechanically, biochemically
and histologically characterized to determine the production of
extracellular matrix and expression of chondrogenic markers. / Ródenas Rochina, J. (2015). Scaffold surface modifications and culture conditions as key parameters to develop cartilage and bone tissue engineering implants [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/48526 / TESIS
| Identifer | oai:union.ndltd.org:upv.es/oai:riunet.upv.es:10251/48526 |
| Date | 31 March 2015 |
| Creators | Ródenas Rochina, Joaquín |
| Contributors | Gómez Ribelles, José Luís, Lebourg, Myriam Madeleine, Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia |
| Publisher | Universitat Politècnica de València |
| Source Sets | Universitat Politècnica de València |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis, info:eu-repo/semantics/acceptedVersion |
| Rights | http://rightsstatements.org/vocab/InC/1.0/, info:eu-repo/semantics/openAccess |
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