Susceptibility to infection with Salmonella varies in different chicken lines suggesting the involvement of host genetic factors. Segregation analysis using Salmonella-resistant and Salmonella-susceptible chickens has shown that early resistance to infection is fully dominant, not sex-linked or associated with the major histocompatibility complex (MHC). In mice, natural resistance to infection with Salmonella typhimurium is controlled by two major loci, Nramp1 and Lps, that exert pleiotropic effects and contribute to cytostatic/cytocidal activities of the macrophage. / We have tested the candidacy of the chicken homologs of Nramp1 and Tnc (a locus closely linked to Lps ) in the differential resistance of chicken inbred lines to infection with Salmonella typhimurium. We first mapped chicken NRAMP1 gene to chromosome 7q13 within a conserved synteny to mouse chromosome 1 and human chromosome 2q. / We have analyzed six inbred chicken lines that were Salmonella -resistant or Salmonella-susceptible for the presence of sequence variation within NRAMP1. Sequence analysis has revealed 11 nucleotide variations within the coding portion of NRAMP1 10 of these sequence variation represent either silent mutations or conservative changes. / We have investigated the effect of NRAMP1 and the chromosomal region surrounding LPS on resistance to infection with S. typhimurium in 425 (CXWI)F1XC chicken progeny. Together, NRAMP1 and TNC explain 33% of the early differential resistance to infection with S. typhimurium of parental lines C and Wl. The data established that both NRAMP1 and TNC contributed to the resistance of chicken to infection with Salmonella typhimurium, and demonstrated that comparative mapping is valuable in identifying Salmonella-resistance genes in the chicken. Furthermore, the results are consistent with the fact that resistance to salmonellosis in chicken is inherited as a complex trait. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.34735 |
| Date | January 1997 |
| Creators | Hu, Jinxin. |
| Contributors | Malo, Danielle (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001604637, proquestno: NQ44455, Theses scanned by UMI/ProQuest. |
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