The diploma work deals with the preparation of biocompatible hydrophilic layers from a propane-butane mixture by plasma polymerization. Subsequent characterization of the layers was performed using both biological (antibacterial tests and biocompatibility test) and physico-chemical diagnostics (SEM, OES and surface energy determination). The theoretical part presents the basic principle of dielectric barrier discharge, its use and various types, including surface dielectric barrier discharge (SDBD), which was used during deposition. Next, cell cultures, cell morphology and clarification of the term biocompatibility were described. At the end of the theoretical part, all used during the experimental part of the diagnostics were also described. In the experimental part, the procedure of deposition of hydrophilic layers and the overall apparatus, which was used during deposition, were presented. Prior to the characterization of the samples, the discharge was diagnosed using optical emission spectroscopy. Furthermore, the level of hydrophilicity of the layers and their elemental composition on the surface was determined. Biological tests for biocompatibility and antibacterial properties provided information on the viability of the cells on the layers and their antibacterial effects.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:449395 |
| Date | January 2021 |
| Creators | Kostyleva, Kseniia |
| Contributors | Trunec, David, Mazánková, Věra |
| Publisher | Vysoké učení technické v Brně. Fakulta chemická |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
Page generated in 0.0024 seconds