Biocatalytic membrane reactors combine the highly efficient biotransformation capability
of enzymes with the selective filtration performance of membrane filters. Common strategies to
immobilize enzymes on polymeric membranes are based on chemical coupling reactions. Still, they
are associated with drawbacks such as long reaction times, high costs, and the use of potentially toxic
or hazardous reagents. In this study, a reagent-free immobilization method based on electron beam
irradiation was investigated, which allows much faster, cleaner, and cheaper fabrication of enzyme
membrane reactors. Two industrial lipase enzymes were coupled onto a polyvinylidene fluoride
(PVDF) flat sheet membrane to create self-cleaning surfaces. The response surface methodology
(RSM) in the design-of-experiments approach was applied to investigate the effects of three numerical
factors on enzyme activity, yielding a maximum activity of 823 118 U m2 (enzyme concentration:
8.4 g L1, impregnation time: 5 min, irradiation dose: 80 kGy). The lipolytic membranes were used in
fouling tests with olive oil (1 g L1 in 2 mM sodium dodecyl sulfate), resulting in 100% regeneration
of filtration performance after 3 h of self-cleaning in an aqueous buffer (pH 8, 37 C). Reusability with
three consecutive cycles demonstrates regeneration of 95%. Comprehensive membrane characterization
was performed by determining enzyme kinetic parameters, permeance monitoring, X-ray
photoelectron spectroscopy, FTIR spectroscopy, scanning electron microscopy, and zeta potential, as
well as water contact angle measurements.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:87526 |
| Date | 20 October 2023 |
| Creators | Schmidt, Martin, Prager, Andrea, Schönherr, Nadja, Gläser, Roger, Schulze, Agnes |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 599 |
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