by Yip Chi Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 70-87). / ABSTRACT --- p.i / ACKNOWLEDGEMENT --- p.iv / TABLE OF CONTENTS --- p.v / LIST OF TABLES --- p.ix / LIST OF FIGURES --- p.xiii / INTRODUCTION --- p.1 / LITERATURE REVIEW --- p.3 / Chapter I. --- Mycobacterial Infections --- p.3 / Chapter A. --- Mycobacterium tuberculosis --- p.3 / Chapter B. --- Atypical mycobacteria --- p.3 / Chapter II. --- Identification of Mycobacteria --- p.5 / Chapter A. --- Conventional methods --- p.6 / Chapter 1. --- Mycobacterium tuberculosis --- p.6 / Chapter 2. --- Atypical mycobacteria --- p.7 / Chapter B. --- Rapid identification methods --- p.8 / Chapter 1. --- Identification by fatty acid analysis --- p.8 / Chapter 2. --- Identification by mycolic acid analysis --- p.9 / Chapter III. --- In vitro Susceptibility Testing of Mycobacteria --- p.11 / Chapter A. --- Mycobacterium tuberculosis --- p.11 / Chapter 1. --- Principle --- p.12 / Chapter 2. --- Methods of susceptibility testing --- p.13 / Chapter a. --- The absolute concentration method --- p.13 / Chapter b. --- The resistance ratio method --- p.15 / Chapter c. --- The 1% proportion method --- p.16 / Chapter d. --- Radiometric method --- p.18 / Chapter e. --- Other methods --- p.19 / Chapter B. --- Atypical mycobacteria --- p.20 / Chapter IV. --- Plasmid Analysis in Mycobacteria --- p.22 / Chapter A. --- Discovery of plasmids in mycobacteria --- p.22 / Chapter B. --- Methodologies in the studies of mycobacterial plasmids --- p.23 / Chapter C. --- Possible roles of plasmid in epidemiology of mycobacteria --- p.24 / MATERIALS AND METHODS --- p.26 / Chapter I. --- Bacterial Strains and Strain Maintenance --- p.26 / Chapter A. --- Strains collection --- p.26 / Chapter B. --- Strains maintenance --- p.26 / Chapter II. --- Culture Media and Culture Conditions --- p.26 / Chapter III. --- Identification of Mycobacteria --- p.26 / Chapter A. --- Conventional methods --- p.26 / Chapter B. --- Fatty acid profile analysis --- p.27 / Chapter 1. --- Bacterial isolates --- p.27 / Chapter 2. --- Standards and reagents --- p.27 / Chapter 3. --- Preparation of methyl ester for GC/GC-MS --- p.28 / Chapter 4. --- Instrumentation --- p.28 / Chapter a. --- Gas chromatography-mass spectrometry (GC-MS) --- p.28 / Chapter b. --- Gas liquid chromatography (GLC) --- p.28 / Chapter 5. --- Fatty acid profile analysis --- p.29 / Chapter a. --- Calibration --- p.30 / Chapter b. --- Identification of mycobacterial fatty acids --- p.30 / Chapter c. --- Construction of mycobacterial fatty acid profiles --- p.30 / Chapter 6. --- Discriminant analysis --- p.31 / Chapter IV. --- In Vitro Drug Susceptibility Test --- p.31 / Chapter A. --- Test strains --- p.31 / Chapter B. --- Preparation of drug-containing media --- p.32 / Chapter C. --- Minmum inhibition concentration (MIC) determination --- p.32 / Chapter V. --- Heavy Metal Tolerance Test --- p.33 / Chapter A. --- Bacterial strains --- p.33 / Chapter B. --- Reagent and media preparation --- p.34 / Chapter 1. --- Heavy metal stock solution preparation --- p.34 / Chapter 2. --- Media preparation --- p.34 / Chapter C. --- Minimum inhibition concentration (MIC) determination --- p.34 / Chapter VI. --- Plasmid Analysis of Mycobacteria --- p.35 / Chapter A. --- Bacterial strains --- p.35 / Chapter B. --- Extraction procedures --- p.35 / Chapter 1. --- Modified Kado & Liu method --- p.35 / Chapter 2. --- French press procedure --- p.36 / Chapter 3. --- Spheroplasts preparation procedure --- p.37 / Chapter C. --- Electrophoresis procedure --- p.37 / Chapter D. --- Statistical analysis for correlation between plasmid and drug resistance or heavy metal tolerance --- p.38 / RESULTS --- p.39 / Chapter I. --- Identification of Mycobacteria --- p.39 / Chapter A. --- General characteristics of the chromatographic profile --- p.39 / Chapter B. --- Discriminant analysis --- p.40 / Chapter 1. --- Gas chromatography-mass spectrometry (GC-MS) --- p.40 / Chapter a. --- Slowly growing non-pigmented mycobacteria --- p.40 / Chapter b. --- Rapidly growing mycobacter-ia --- p.41 / Chapter c. --- Pigmented mycobacteria --- p.41 / Chapter 2. --- Gas liquid chromatography (GLC) --- p.41 / Chapter a. --- Slowly growing non-pigmented mycobacteria --- p.42 / Chapter b. --- Rapidly growing mycobacteria --- p.42 / Chapter c. --- Pigmented mycobacteria --- p.43 / Chapter II. --- Vitro Drug Susceptibility Test --- p.43 / Chapter A. --- Mycobacterium tuberculosis --- p.43 / Chapter B. --- Atypical mycobacteria --- p.45 / Chapter 1. --- General characteristics --- p.45 / Chapter 2. --- Sensitivity pattern of different species --- p.46 / Chapter a. --- Mycobacterium kansasii --- p.46 / Chapter b. --- Mycobacterium avium- intracellulare complex --- p.46 / Chapter c. --- Mycobacterium scrofulaceum --- p.47 / Chapter d. --- Mycobacterium terrae complex --- p.47 / Chapter e. --- Mycobacterium fortuitum --- p.47 / Chapter f. --- Mycobacterium chelonae --- p.48 / Chapter III. --- Heavy Metal Tolerance Test --- p.48 / Chapter IV. --- Plasmid in Mycobacteria --- p.48 / Chapter A. --- Mycobacterium tuberculosis --- p.48 / Chapter B. --- Atypical mycobacteria --- p.49 / Chapter 1. --- General characteristics --- p.49 / Chapter 2. --- Correlation between drug resistance and plasmid --- p.50 / Chapter 3. --- Correlation between heavy metal tolerance and plasmid --- p.50 / DISCUSSION --- p.52 / Chapter I. --- Identification of Mycobacteria --- p.52 / Chapter II. --- In Vitro Drug Susceptibility Test --- p.56 / Chapter A. --- Mycobacterium tuberculosis --- p.56 / Chapter B. --- Atypical mycobacteria --- p.60 / Chapter 1. --- Mycobacterium kansasii --- p.61 / Chapter 2. --- Mycobacterium avium- intracellulare complex --- p.61 / Chapter 3. --- Mycobacterium scrofulaceum --- p.62 / Chapter 4. --- Mycobacterium terrae complex --- p.62 / Chapter 5. --- Mycobacterium fortuitum --- p.63 / Chapter 6. --- Mycobacterium chelonae --- p.64 / Chapter III. --- Plasmid Analysis in Mycobacteria --- p.64 / Chapter A. --- Mycobacterium tuberculosis --- p.64 / Chapter B. --- Atypical mycobacteria --- p.66 / SUMMARYS AND CONCLUSIONS --- p.68 / LITERATURE CITED --- p.70 / Chapter APPENDIX - --- Tables --- p.88 / Figures --- p.144
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318918 |
| Date | January 1992 |
| Contributors | Yip, Chi Wai., Chinese University of Hong Kong Graduate School. Division of Clinical and Pathological Sciences. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xv, 176 leaves, [33] leaves of plates : ill. (some mounted) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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