Melanomas account for more than 1.7% of global cancer diagnoses and about 1% of all skin cancer diagnoses in the United States. This type of cancer occurs in the melanin-producing cells in the epidermis and exhibits distinctive variations in melanin and blood concentration values in the form of skin lesions. The current approach for evaluating skin cancer lesions involves visual inspection with a dermatoscope, typically followed by biopsy and histopathological analysis. However, this process, to decrease the risk of misdiagnosis, results in unnecessary biopsies, contributing to the emotional and financial distress of patients. The implementation of a non-invasive imaging technique to aid the analysis of skin lesions in the early stages can potentially mitigate these consequences.
Hyperspectral imaging (HSI) has shown promise as a non-invasive technique to analyze skin lesions. Images taken of human skin using a hyperspectral camera are a result of numerous elements in the skin. Being a turbid, inhomogeneous material, the skin has chromophores and scattering agents, which interact with light and produce characteristic back-scattered energy that can be harnessed and examined with an HSI camera. In this study, a mathematical model of the skin is used to extract meaningful information from the hyperspectral data in the form of melanin concentration, blood volume fraction and blood oxygen saturation in the skin. The human skin is modelled as a bi-layer planar system, whose surface reflectance is theoretically calculated using the Kubelka-Munk theory and absorption laws by Beer and Lambert. Hyperspectral images of the dorsal portion of three volunteer subjects' hands 400 - 1000 nm range, were used to estimate the contributing parameters. The mean and standard deviation of these estimates are reported compared with theoretical values from the literature. The model is also evaluated for its sensitivity with respect to these parameters, and then fitted to measured hyperspectral data of three volunteer subjects in different conditions. The wavelengths and wavelength groups which were identified to result in the maximum change in percentage reflectance calculated from the model were 450 and 660 nm for melanin, 500 - 520 nm and 590 - 625 nm for blood volume fraction and 606, 646 and 750 nm for blood oxygen saturation. / Master of Science / Melanoma, the most serious type of skin cancer, develops in the melanin-producing cells in the epidermis. A characteristic marker of skin lesions is the abrupt variations in melanin and blood concentration in areas of the lesion. The present technique to inspect skin cancer lesions involves dermatoscopy, which is a qualitative visual analysis of the lesion's features using a few standardized techniques such as the 7-point checklist and the ABCDE rule. Typically, dermatoscopy is followed by a biopsy and then a histopathological analysis of the biopsy. To reduce the possibility of misdiagnosing actual melanomas, a considerable number of dermoscopically unclear lesions are biopsied, increasing emotional, financial, and medical consequences. A non-invasive imaging technique to analyze skin lesions during the dermoscopic stage can help alleviate some of these consequences.
Hyperspectral imaging (HSI) is a promising methodology to non-invasively analyze skin lesions. Images taken of human skin using a hyperspectral camera are a result of numerous elements in the skin. Being a turbid, inhomogeneous material, the skin has chromophores and scattering agents, which interact with light and produce characteristic back-scattered energy that can be harnessed and analyzed with an HSI camera. In this study, a mathematical model of the skin is used to extract meaningful information from the hyperspectral data in the form of melanin concentration, blood volume fraction and blood oxygen saturation. The mean and standard deviation of these estimates are reported compared with theoretical values from the literature. The model is also evaluated for its sensitivity with respect to these parameters, and then fitted to measured hyperspectral data of six volunteer subjects in different conditions. Wavelengths which capture the most influential changes in the model response are identified to be 450 and 660 nm for melanin, 500 - 520 nm and 590 - 625 nm for blood volume fraction and 606, 646 and 750 nm for blood oxygen saturation.
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/116069 |
| Date | 21 August 2023 |
| Creators | Karambor Chakravarty, Sriya |
| Contributors | Mechanical Engineering, Wicks, Alfred L., Southward, Steve C., Abbott, Amos L. |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | ETD, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
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