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Genetical studies of the cultivated mushroom Lentinus edodes.

by Chan Ngar Lei Annie. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 90-102. / ACKNOWLEDGEMENTS --- p.i / ABSTRACT --- p.ii / TABLE OF CONTENTS --- p.iv / LIST OF FIGURES --- p.vii / LIST OF TABLES --- p.ix / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 2. --- LITERATURE REVIEW --- p.5 / Chapter 2.1 --- Economic and Biotechnological Significance of Lentinus edodes --- p.5 / Chapter 2.1.1 --- General Review --- p.5 / Chapter 2.1.2 --- Nutritional and Medicinal Values --- p.7 / Chapter 2.1.3 --- Lignocellulose Degradation and Utilization --- p.9 / Chapter 2.2 --- Biological Background --- p.11 / Chapter 2.2.1 --- Life Cycle --- p.11 / Chapter 2.2.2 --- Patterns of Sexuality --- p.12 / Chapter 2.3 --- Genetic Improvement of Lentinus edodes --- p.16 / Chapter 2.3.1 --- Introduction --- p.16 / Chapter 2.3.2 --- Mutagenic Agents --- p.17 / Chapter 2.3.3 --- Genetic Markers and Strain Improvement --- p.20 / Chapter 3. --- MATERIALS AND METHODS --- p.24 / Chapter 3.1 --- Biological Materials --- p.24 / Chapter 3.2 --- Media --- p.25 / Chapter 3.2.1 --- Complete Medium (CM) --- p.25 / Chapter 3.2.2 --- Complete Medium with Yeast Extract (CM with Y.E.) --- p.25 / Chapter 3.2.3 --- Complete Fruiting Medium (CF) --- p.25 / Chapter 3.2.4 --- Complete Migration Medium (CMM) --- p.25 / Chapter 3.2.5 --- Minimal Medium (MM) --- p.25 / Chapter 3.2.6 --- Carboxymethyl Cellulose - Leatham Medium (CMC - Leatham) --- p.26 / Chapter 3.2.7 --- Potato Dextrose Agar (PDA) --- p.26 / Chapter 3.3 --- Anti-metabolites for Screening of Resistant Mutants --- p.27 / Chapter 3.4 --- Characterization of Monokaryons --- p.28 / Chapter 3.4.1 --- Isolation of Monosporous Mycelia --- p.28 / Chapter 3.4.2 --- Assessment of Mycelial Growth --- p.28 / Chapter 3.4.3 --- Determination of Mating Type of Monosporous Mycelia --- p.28 / Chapter 3.5 --- Mutagenesis and Isolation of Mutants --- p.29 / Chapter 3.5.1 --- Effects of Homogenization on Growth --- p.29 / Chapter 3.5.2 --- Determination of the Ultraviolet Irradiation Killing Curve --- p.29 / Chapter 3.5.3 --- Isolation of High Temperature Tolerant Strains --- p.30 / Chapter 3.5.4 --- Isolation of Auxotrophic Mutants --- p.30 / Chapter 3.5.5 --- Isolation of Anti-metabolite Resistant Mutants --- p.33 / Chapter 3.6 --- Characterization of Anti-metabolite Resistant Mutants --- p.33 / Chapter 3.6.1 --- "Measurements of Growth Rate, Anti-metabolites Resistance and Osmotic Sensitivity" --- p.33 / Chapter 3.6.2 --- Determination of Dominance Relationships --- p.34 / Chapter 3.6.3 --- Detection of Extracellular Enzymes --- p.34 / Chapter 4. --- RESULTS --- p.36 / Chapter 4.1 --- Radial Growth Rate of Monosporous Cultures --- p.36 / Chapter 4.2 --- Mating Reactions in Lentinus edodes --- p.36 / Chapter 4.3 --- Effects of Homogenization on Growth of L. edodes --- p.46 / Chapter 4.4 --- Determination of Ultraviolet (UV) Killing Curve --- p.46 / Chapter 4.5 --- Selection of High Temperature Tolerant Strains --- p.50 / Chapter 4.6 --- Auxotrophic Mutants Isolation and Identification --- p.50 / Chapter 4.7 --- Selection of Anti-metabolite Resistant Mutants --- p.52 / Chapter 4.8 --- Characterization of Cycloheximide Resistant Mutants V --- p.63 / Chapter 4.8.1 --- "Growth Rate, Anti-metabolite Resistance and Osmotic Sensitivity of Cycloheximide Resistant Mutants" --- p.63 / Chapter 4.8.2 --- Dominance Tests for Cycloheximide Resistant Strains --- p.66 / Chapter 4.8.3 --- Detection of Extracellular Enzymes --- p.73 / Chapter 5. --- DISCUSSION --- p.78 / Chapter 5.1 --- Mating Reactions in Lentinus edodes --- p.78 / Chapter 5.2 --- Isolation of Auxotrophic and Anti-metabolites Resistant Mutants --- p.81 / Chapter 5.3 --- Characterization of Cycloheximide Resistant Mutants of Lentinus edodes --- p.84 / CONCLUSION --- p.88 / REFERENCES --- p.90

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318603
Date January 1990
ContributorsChan, Ngar Lei., Chinese University of Hong Kong Graduate School. Division of Biology.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, ix, 102 leaves ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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