6 Abstract This thesis focuses on the identification and quantification of volatile metabolites in the exhaled breath that might be used as possible biomarkers of Gastroesophageal Reflux Disease. Animal tissue samples were exposed to conditions simulating the gastric environment to identify specific volatile compounds that would be chosen for real-time quantification in exhaled breath of GERD patients and healthy controls using selected ion flow tube mass spectrometry. Solid phase microextraction, was used in combination with gas chromatography mass spectrometry, for qualitative analyses of the headspace of these samples. Only acetic acid was significantly elevated and so it has been elected for the quantitative analysis in the breath of the patients. The median concentration of acetic acid measured by SIFT-MS in the exhaled breath of twenty-two GERD patients was found to be higher (85 ppbv) in comparison to the control group (31 ppbv). The results show that breath acetic acid could be valuable marker for GERD diagnosis and monitoring.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:331989 |
| Date | January 2014 |
| Creators | Pospíšilová, Veronika |
| Contributors | Španěl, Patrik, Jelínek, Ivan |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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