<p>Skeletal muscle takes up nearly 40% of total body mass, is critical for daily function by</p>
<p>providing balance, supports breathing, movement, and energy expenditure. Preserving</p>
<p>skeletal muscle can also significantly improve one’s quality by maintaining balance, movement</p>
<p>and improving metabolic health [1, 2]. This becomes more imperative with age, as skeletal muscle mass naturally declines, and further compounds decline in quality of life and health [1, 2]. Thus, it is critical to understand the physiology of skeletal muscle and the underlying cellular and</p>
<p>molecular mechanisms that contribute to normal function. Using mouse models to further our</p>
<p>understanding, this dissertation leverages single-cell RNA-sequencing (scRNA-seq) to dissect the</p>
<p>cellular and molecular underpinnings of skeletal muscle injury and repair. Specifically, chapter 1</p>
<p>provides an overview of skeletal muscle structure, muscle regeneration, and the current state of</p>
<p>scRNA-seq literature in muscle regeneration. In chapter 2, I will discuss the large-scale scRNAseq of regenerating muscle which identified dynamic population of resident and infiltrating cells. In chapter 3, I will discuss the potential immunomodulatory role of MuSCs and leveraging scRNAseq data to understand the cellular mechanisms that govern successful muscle regeneration. Finally, in chapter 4 I will discuss the role of the transcription factor Sox11, which was identified by scRNA-seq and was specific to differentiating MuSCs. Thus, this dissertation spans the cellular and molecular components of muscle regeneration.</p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/22572571 |
| Date | 10 April 2023 |
| Creators | Stephanie Oprescu (15195469) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/thesis/_20230328_SOPRESCU-Dissertation_pdf/22572571 |
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