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Previous issue date: 2017-12-26 / Introduction: endometrial cancer is the most prevalent gynecological neoplasm in developed countries and the second most prevalent in Brazil, second only to cervical cancer. It corresponds to 5% of cancers in women worldwide. The majority of cases present as initial disease, and surgical treatment is curative. Few treatment options exist for patients with poor prognostic factors and advanced disease, and the process of carcinogenesis in endometrial cancer has not been fully elucidated. Sirtuins are proteins involved in several cellular processes. The family of these proteins is composed of seven sirtuins (SIRT1-SIRT7). They present specific cell locations, acting in a variety of processes, including regulation of gene transcription, genomic stability, metabolic control, cell cycle regulation, and cell survival. Few studies have evaluated the role of sirtuins in endometrial cancer as well as its association with prognostic factors. Objective: to evaluate the expression of SIRT1 and SIRT7 enzymes in histological samples of endometrial carcinoma of patients operated at Hospital S?o Lucas (Pontif?cia Universidade Cat?lica) between January 2000 and December 2015 and associate the expression of these proteins with the pathological staging and prognostic factors. Materials and Methods: we performed a retrospective study evaluating 66 samples of patients with endometrial cancer from January 2000 to December 2015. The expression of SIRT1 and SIRT7 proteins was identified by the immunohistochemical technique and quantified by the Allred score. The expression of these proteins in cancer samples was correlated with the prognostic factors: age, tumor grade, tumor size, myometrial invasion, lymphovascular invasion and pathological staging. Results: SIRT 1 expression was positive in 40.9% of samples and SIRT7 expression in 24.1% of cases. Expression of these proteins did not present a statistically significant association with any of the adverse prognostic factors in endometrial cancer. Conclusions: endometrial cancer samples were positive for both SIRT1 and SIRT7, but without correlation in the study with prognostic factors. Expression of these proteins in samples of endometrial cancer may represent a possible therapeutic target to be developed in future studies. / Introdu??o: O c?ncer de endom?trio ? a neoplasia maligna ginecol?gica mais prevalente nos pa?ses desenvolvidos e a segunda mais prevalente no Brasil, menos freq?ente apenas do que o c?ncer de colo uterino. Corresponde a 5% dos c?nceres em mulheres no mundo. A maioria dos casos se apresenta como doen?a inicial, sendo o tratamento cir?rgico curativo. Existem poucas op??es de tratamento para pacientes com fatores progn?sticos adversos e com doen?a avan?ada, sendo ainda pouco elucidado o processo de carcinog?nese no c?ncer de endom?trio. As sirtu?nas s?o prote?nas envolvidas em diversos processos celulares. Sua fam?lia ? composta por sete sirtu?nas (SIRT1-SIRT7). Apresentam localiza??es celulares espec?ficas, atuando em uma s?rie de processos, incluindo a regula??o da transcri??o g?nica, estabilidade gen?mica, controle metab?lico, regula??o do ciclo celular e sobreviv?ncia celular. Poucos estudos avaliaram o papel das sirtu?nas no c?ncer de endom?trio, bem como a sua associa??o com fatores progn?sticos. Objetivo: avaliar a express?o das enzimas SIRT1 e SIRT7 em amostras histol?gicas de carcinoma de endom?trio de pacientes operadas no Hospital S?o Lucas da Pontif?cia Universidade Cat?lica do Rio Grande do Sul (PUCRS) no per?odo de janeiro de 2000 a dezembro de 2015 e associar a express?o dessas prote?nas com o estadiamento patol?gico e fatores progn?sticos. M?todos: foi realizado um estudo retrospectivo com a avalia??o de 66 amostras de pacientes com c?ncer de endom?trio no per?odo de janeiro de 2000 at? dezembro de 2015. A express?o das prote?nas SIRT1 e SIRT7 foi identificada pela t?cnica de imunohistoqu?mica e quantificada pelo escore de Allred. A express?o dessas prote?nas nas amostras de neoplasia foi correlacionada com os fatores progn?sticos: idade, grau tumoral, tamanho do tumor, invas?o miometrial, invas?o linfovascular e estadiamento patol?gico. Resultados: a express?o de SIRT1 foi positiva em 40.9% das amostras e a de SIRT7 em 24.1% dos casos. A express?o dessas prote?nas n?o apresentou associa??o estatisticamente significativa com nenhum dos fatores progn?sticos adversos no c?ncer de endom?trio. Conclus?es: as amostras de c?ncer de endom?trio apresentaram positividade, tanto para SIRT1 quanto para SIRT7, mas sem correla??o com fatores progn?sticos. A express?o dessas prote?nas em amostras de neoplasia de endom?trio pode significar um poss?vel alvo terap?utico a ser estudado e desenvolvido em estudos futuros.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/8029 |
| Date | 26 December 2017 |
| Creators | Oppermann, Christina Pimentel |
| Contributors | Carvalhal, Gustavo Franco |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, Brasil, Escola de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 500, 500, 500, -224747486637135387, -969369452308786627 |
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