Systemic Lupus Erythematosus (SLE) is an autoimmune disorder associated with the development of immune complexes that deposit in multiple organ systems. The deposition of immune complexes is associated with tissue damage, most commonly seen in the form of kidney damage as lupus nephritis. IRF5 is an SLE susceptibility gene that is causal to type one interferon inflammatory cytokine production. We hypothesized that the peak luminescence of Donkey anti-mouse IgG Alexa-594 and for Goat anti-mouse C3-FITC in FcγRIIB-/- Yaa mice can show the disease state of SLE in murine models at which disease is most active. This thesis demonstrates that peak luminosity in FcγRIIB-/- Yaa mice is at 4.5 months of age. IHC staining was done to show the deposition of IgG and C3 protein in the glomeruli of FcγRIIB-/- Yaa at various ages. It also shows that the disease manifests between the ages of 2-4.5 months of age due to the differences in fluorescence intensity.
| Identifer | oai:union.ndltd.org:bu.edu/oai:open.bu.edu:2144/43537 |
| Date | 10 December 2021 |
| Creators | Rowley, Rachael |
| Contributors | Rifkin, Ian, Symes, Karen |
| Source Sets | Boston University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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