This study investigates the effect on: 1) the bulk surface; and 2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacylamide)-CNT-polyaniline on growth and viability of mice fibroblast cells L929. The poly(N-isopropylacylamide)-CNT-polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, nonwoven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400-500 mm; L: 6-20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacylamide)-CNT-polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacylamide)-CNT-polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacylamide)-CNT-polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behaviour was observed on the poly(N-isopropylacylamide)-CNT-polyaniline scaffolds by varying incubation at below lower critical solution temperature (LCST) of poly(N-isopropylacylamide). The results suggest that poly(N-isopropylacylamide)-CNT-polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering. / European Commission FP7 (PIIF-GA-2009-254955), JSPS, JST-CREST and MEXT
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:liu-85583 |
| Date | January 2013 |
| Creators | Tiwari, Ashutosh, Sharma, Yashpal, Hattori, Shinya, Terada, Dohiko, Sharma, Ashok K., Turner, Anthony P. F., Kobayashi, Hisatoshi |
| Publisher | Linköpings universitet, Biosensorer och bioelektronik, Linköpings universitet, Tekniska högskolan, Linköpings universitet, Biosensorer och bioelektronik, Linköpings universitet, Tekniska högskolan, National Institute for Materials Science, Japan, National Institute for Materials Science, Japan, National Institute for Materials Science, Japan, DCR University of Science and Technology, India, National Institute for Materials Science, Japan |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Article in journal, info:eu-repo/semantics/article, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | Biopolymers, 0006-3525, 2013, 99:5, s. 334-341, info:eu-repo/grantAgreement/EC/FP7/PIIF-GA-2009- 254955 |
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