The cadA gene in Dictyostelium encodes a unique Ca2+-dependent cell adhesion molecule DdCAD-1. It is synthesized as a soluble protein in the cytoplasm and then transported to the plasma membrane by contractile vacuoles. The solution structures of Ca2+-free and Ca2+-bound DdCAD-1 reveals that it contains two β-sandwich domains, belonging to the βγ-crystallin and immunoglobulin fold classes, respectively. Whereas the N-terminal domain has a major role in homophilic binding, the C-terminal domain tethers the protein to the cell membrane. Although hydrophobic interactions constitute the major force for adhesion, electrostatic interactions may act as a ‘switch’ to regulate the homophilic binding by a change in electrostatic potential caused by the binding of Ca2+ to the three binding sites.
To further investigate DdCAD-1 transport, DdCAD-1-GFP fusion proteins were expressed in cadA-null cells. Time-lapse microscopy revealed that DdCAD-1 was imported by invagination of the contractile vacuole membrane. The N-terminal, C-terminal domains, and two of the three Ca2+-binding site mutant forms of DdCAD-1 failed to enter the contractile vacuole, suggesting that Ca2+-binding and the integrity of DdCAD-1 are required for import. Indeed, proteins with altered conformation failed to enter the contractile vacuole, indicating that the import signal is integrated in the three-dimensional structure of DdCAD-1.
Finally, we describe how the cadA gene acts as a single-gene green beard. In chimera experiments, cells expressing DdCAD-1 were more likely to form fruiting bodies than cadA-null cells on soil plates. Here cadA behaved as a single gene green beard. However, cadA exhibited anti-green beard behaviour on non-nutrient agar plates. Wild-type cells differentiated mostly into prestalk cells and eventually died, whereas the cadA-null cells survived as spores. DdCAD-1 was enriched in cell-cell contact regions of anterior cells, while it was mostly localized in the cytoplasm of posterior cells. The presence of DdCAD-1 on the cell surface of prestalk cells is crucial for cell sorting, which in turn explain the anti-green beard effect observed in chimeras containing cadA+ and cadA- cells. These observations demonstrate that DdCAD-1 plays a direct role in cell sorting through differential cell-cell adhesion which results from the differential distribution of DdCAD-1.
Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/29877 |
Date | 31 August 2011 |
Creators | Sriskanthadevan, Shrivani |
Contributors | Siu, Chi-Hung |
Source Sets | University of Toronto |
Language | en_ca |
Detected Language | English |
Type | Thesis |
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