This research investigate the matrix effect, operation time, cost and solvent comsumption for triarylmethanes and corresponding metabolites in different aquaculture products by different extraction methods. Triarylmethane was determined by LC-ESI-MS/MS in positive mode. The analytical method validation were followed the regulation of 2002/657/EC. HPLC separation was conducted with the RP-18 column. The mobile phases consisted of 0.5 mM ammonium acetate buffer (pH 3.8, adjusted with acetic acid) ¡V acetonitrile (contained 0.1% formic acid) solution.
The ratios of standard deviation to average residual time for all compounds are less than 0.05. The ion ratio is stable and allowed by the regulation of 2002/657/EC. The regression constant of calibration curve is in the range from 0.998 to 0.999. The CC£\ for MG, LMG, CV, LCV are 0.09 ¡Ó 0.05, 0.19 ¡Ó 0.14, 0.15 ¡Ó 0.13, 0.15 ¡Ó 0.10 £ggkg-1, respectively. The CC£] for MG, LMG, CV, LCV are 0.09 ¡Ó 0.05, 0.19 ¡Ó 0.14, 0.15 ¡Ó 0.13, 0.15 ¡Ó0.10 £ggkg-1, respectively.
The results for SPE and liquid-liquid extraction are not significantly different; the liquid-liquid extraction have shorter operation time, cheaper cost and less solvent consumption in comparison with SPE.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0901110-165517 |
| Date | 01 September 2010 |
| Creators | Hsiang, Huang-Sung |
| Contributors | Jung-Hui Chen, Wei-Hsien Wang, Chi-Hsin Hsu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0901110-165517 |
| Rights | off_campus_withheld, Copyright information available at source archive |
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