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Application of WATR-CPMG method to the quantitative determination of glucose in dilute aqueous solutions.

by Choy Wing Yiu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 115-121). / DESCRIPTIVE NOTE --- p.iii / ACKNOWLEDGEMENTS --- p.v / Chapter CHAPTER ONE: --- BRIEF REVIEW OF THE WATR-CPMG AND OTHER SOLVENT SUPPRESSION METHODS --- p.1 / Chapter 1.0 --- Introduction --- p.1 / Chapter 1.1 --- Some Common Solvent Suppression Techniques --- p.2 / Chapter 1.1.1 --- Brief Summary of Some Common Solvent Suppression Techniques --- p.2 / Chapter 1.1.2 --- Criteria for the Evaluation of Solvent Suppression Methods --- p.8 / Chapter 1.2 --- Application of the WATR-CPMG Method in Solvent Suppression --- p.10 / Chapter 1.2.1 --- The CPMG Pulse Sequence --- p.10 / Chapter 1.2.2 --- The Theory of Solvent Suppression by WATR Method --- p.12 / Chapter 1.3 --- Scope of this Thesis --- p.15 / Chapter CHAPTER TWO: --- OPTIMIZATION OF WATR-CPMG PARAMETERS FOR QUANTITATIVE ANALYSIS IN DILUTE AQUEOUS SOLUTION --- p.19 / Chapter 2.1 --- Investigation of the WATR-CPMG Parameters --- p.19 / Chapter 2.1.1 --- Use of Ammonium Chloride as Proton Exchange Reagent --- p.19 / Chapter 2.1.2 --- The pH Dependence of the Proton Exchange Process --- p.21 / Chapter 2.1.3 --- Relaxation Delay Time --- p.27 / Chapter 2.1.4 --- Measurement of T1 of Protons In Aqueous Solution --- p.31 / Chapter 2.1.5 --- The position of the Carrier Frequency --- p.41 / Chapter 2.1.6 --- Modelling of the Off-Resonance Effect --- p.46 / Chapter 2.1.7 --- The Tau Value Used in the WATR-CPMG Method --- p.51 / Chapter 2.2 --- The Setting of Acquisition Parameters and Data Processing --- p.57 / Chapter 2.2.1 --- Digital Resolution and Spectral Width --- p.57 / Chapter 2.2.2 --- The Setting of Receiver Gain --- p.60 / Chapter 2.3 --- Summary --- p.61 / Chapter CHAPTER THREE: --- APPLICATION OF THE WATR-CPMG METHOD TO THE QUANTITATIVE ANALYSIS OF GLUCOSE IN BLOOD PLASMA AND DILUTE AQUEOUS SOLUTION --- p.62 / Chapter 3.0 --- Introduction --- p.62 / Chapter 3.1 --- Experimental Section --- p.62 / Chapter 3.1.1 --- Characterization of the Metabolites in Blood Plasma --- p.62 / Chapter 3.1.2 --- Reagents --- p.66 / Chapter 3.1.3 --- Solution Preparations --- p.66 / Chapter 3.1.4 --- Measurement of Plasma Glucose by Glucose Oxidase Method --- p.67 / Chapter 3.1.5 --- NMR Measurement --- p.68 / Chapter 3.1.6 --- HPLC Measurement --- p.78 / Chapter 3.2 --- Results and Discussion --- p.79 / Chapter 3.2.1 --- Analysis of Results --- p.79 / Chapter 3.2.2 --- Discussion --- p.84 / Chapter 3.2.3 --- Conclusion --- p.85 / Chapter CHAPTER FOUR: --- HOMODECOUPLED WATR-CPMG METHOD --- p.86 / Chapter 4.0 --- Introduction --- p.86 / Chapter 4.1 --- Application of Combined Time Sharing Homodecoupling WATR-CPMG Method for Quantitative Analysis in Aqueous Solution --- p.87 / Chapter 4.1.1 --- The Time Sharing Homonuclear Decoupling and Average Hamiltonian Theory --- p.87 / Chapter 4.1.2 --- Homodecoupling in Aqueous Solution --- p.89 / Chapter 4.2 --- Experimental --- p.90 / Chapter 4.2.1 --- The Preliminary Investigation --- p.90 / Chapter 4.2.2 --- Homodecoupl ing in 30%D20/70%D20 Solution --- p.97 / Chapter 4.2.3 --- Homodecoupling-CPMG (HDCPMG) in Quantitative Analysis --- p.102 / Chapter 4.3 --- Conclusion --- p.110 / Chapter CHAPTER FIVE: --- CONCLUSION --- p.113 / BIBLIOGRAPHY --- p.115 / APPENDIX I The Computer Simulation of the Off-Resonance Effect on the Water Signal Suppression --- p.122 / APPENDIX II The Computer Simulation Program --- p.125 / APPENDIX III The Simulation Data --- p.129

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_319205
Date January 1993
ContributorsChoy, Wing Yiu., Chinese University of Hong Kong Graduate School. Division of Chemistry.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xvii, 132 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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