It was postulated that 3D cell culture models more accurately reflect the complex tissue
physiology and morphology in comparison to 2D cell monolayers. Currently, there is a shortage
of well-characterized and easily maintainable high-throughput experimental models of the human
placenta. Here, we characterized three different 3D cultures (e.g., spheroids) derived from trophoblast
cell lines and studied their functionality in comparison to primary fetal trophoblasts and placental
tissue. The spheroid growth rates of JEG3, BeWo and HTR8/SVneo cell lines were similar among each
other and were significantly larger in comparison to primary trophoblast spheroids. All spheroids
exhibited migratory properties and shortest distances were registered for JEG3 spheroids. Even
though all spheroids displayed invasive capabilities, only the invasive features of HTR8/SVneo
spheroids resulted in specific branching. This was in agreement with the invasive properties of the
spheroids obtained from primary trophoblasts. Human chorionic gonadotropin production was
highest in JEG3 spheroids and only increased when stimulated with cAMP and forskolin in BeWo, but
not HTR8/SVneo spheroids. The gene expression analysis confirmed that 3D trophoblast cell cultures
and especially HTR8/SVneo spheroids showed considerable similarities with the gene expression
profile of primary placental tissue. This study offers a broad characterization of 3D trophoblast
spheroids that, in turn, can help in selecting the best model depending on the scientific question that
needs to be answered.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:92802 |
| Date | 26 July 2024 |
| Creators | Stojanovska, Violeta, Arnold, Susanne, Bauer, Mario, Voss, Hermann, Fest, Stefan, Zenclussen, Ana Claudia |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2073-4409, 2884, 10.3390/cells11182884 |
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