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Physiology and morphology of epithelia in the freshwater demosponge, Spongilla lacustris

Epithelia form protective barriers and regulate molecule transport between the mesenchyme and environment. Amongst all metazoans, only sponges are said to lack 'true' epithelia however the physiology of sponge cell layers are rarely studied empirically. Aggregates and gemmules of a freshwater demosponge, Spongilla lacustris, were used to grow confluent tissue over permeable culture wells which are required for transepithelial recordings. The transepithelial potential (TEP) of S. lacustris was slightly negative (-3mV), indicating possible control of ion transport. Transepithelial resistance (TER) was recorded between 1-2 k cm2, the same order of magnitude as many vertebrate epithelia. Cultures with high resistance blocked the passage of the small tracer molecules 14C-PEG, 3H-Inulin and ruthenium red. Pinacocytes were spatially stable over time and epithelial layers were morphologically similar in freshwater and marine species. These results suggest that sponge cell layers are able to control solute and ion transport, the physiological attributes of functional epithelia. / Physiology, Cell and Development Biology

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/1411
Date11 1900
CreatorsAdams, Emily
ContributorsLeys, Sally (Biological Sciences), Leys, Sally (Biological Sciences), Goss, Greg (Biological Sciences), Gallin, Warren (Biological Sciences), Cheeseman, Chris (Physiology)
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeThesis
Format5430139 bytes, application/pdf
RelationLeys, S. P., Nichols, S. A. & Adams, E. D. M. (2009) Epithelia and integration in sponges. Integr. Comp. Biol. 49: 167-177.

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