There are a lot of inflammatory agents, such as substance P, histamine, capsaicin, and lipopolysaccharide¡]LPS¡^. LPS is the component of all gram-negative bacterial cell wall that can stimulate the immune cells to release pro-inflammatory cytokines that can induce systemic acute inflammation and sepsis. The present study sought to investigate the location of leaky microvessels and magnitude of plasma leakage in the rat stomach secretory and non-secretory portions after an intravenous injection of high dose of LPS¡]15 mg/kg¡^. India ink¡]1 ml/kg¡^ and Evans blue¡]30 mg/ml¡^were used as tracer dyes to measure the magnitude of plasma leakage after LPS application. In the whole mounts of the stomach secretory and non-secretory portions with silver staining, the boundaries of endothelial cells and gaps between endothelial cells for plasma leakage in the blood vessels of microcirculation were made visible. Tissue sections were stained with Alcian blue and periodic acid-Schiff reagent to reveal the mucosubstance present in the mucous cells of the stomach secretory tissue.
The result of study demonstrated that the magnitude of LPS-induced plasma leakage in the rat stomach secretory and non-secretory portions was larger than that of the vehicle control. Extensive plasma extravasation was found from 5 min to 30 min after LPS injection. Numerous endothelial gaps formed in both postcapillary venules and collecting venules in the rat stomach secretory portion at 5 min and 30 min after LPS, but the number of gaps declined strikingly 60 min after LPS. Endothelial gaps were rare in the stomach of vehicle control. Histological sections of the stomach secretory portion showed that the leaky vessels were present in the serosa and muscularis externa. Administration of LPS also resulted in release of mucus in gastric gland cells. It is concluded that endotoxin-induced increase in plasma leakage correlated with the formation of endothelial gaps, and associated with depletion of mucosubstance from the mucous cells.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0717103-192917 |
| Date | 17 July 2003 |
| Creators | Lin, Li-Ling |
| Contributors | Ming-Hung Tai, Jau-Cheng Liou, Hung-Tu Hwang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0717103-192917 |
| Rights | unrestricted, Copyright information available at source archive |
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