Thesis (MSc)--Stellenbosch University, 2003. / ENGLISH ABSTRACT: A laboratory-scale model was used to simulate biological sulfate removal. The
focus of the research was microbial community response, such as the relative
abundance of functional groups to changes in influent medium composition.
Specific oligonucleotide probes were obtained that recognised sulfate
reducing bacteria (SRB) within the biofilm community. Terminal restriction
fragment length polymorphism (T-RFLP) and BIOLOG™ Ecoplate analyses
were used to study the SRB community when provided with sodium lactate,
sucrose or ethanol as carbon sources in complex Postgate C broth. These
two analyses, as well as conventional methods, were applied to follow
succession patterns in the laboratory scale reactors, and to determine the
possible presence and relative abundance of microorganisms other than
bacteria under sulfate reducing conditions. T-RFLP and BIOLOG™ Ecoplate
analyses indicated a few dominant organisms in the community and a slight
decline after a shift to another carbon source. Fluorescent hybridization
showed higher numbers of SRB relative to the total microbial community than
conventional culturing techniques. Furthermore, microscopic observations
showed that not only SRB and other bacteria, but also yeast and filamentous
fungi were integrated in a biofilm under sulfate reducing conditions. These
microscopic observations were verified with fluorescent in situ hybridization
(FISH) and yeast Live I Dead viability probes. / AFRIKAANSE OPSOMMING: Biologiese sulfaat-verwydering is met behulp van "n laboratoriumskaalmodel
gesimuleer. Die doel van die navorsing was om die respons van "n mikrobiese
gemeenskap met byvoorbeeld die relatiewe hoeveelheid van funksionele
groepe op veranderinge in invloeiende medium samestelling te bestudeer.
Spesifieke oligonukleotiedpeilers wat sulfaatreduserende bakterieë (SRB) in
"n biofilmgemeenskap kan opspoor is gebruik. Die SRB gemeenskap is
bestudeer met behulp van terminale-restriksiefragmentlengtepolimorfisme (TRFLP)
en BIOLOGTM Ecoplate analise waar natriumlaktaat, sukrose of etanol
as koolstofbronne toegevoeg is. Hierdie twee tipes analise en konvensionele
metodes is aangewend om suksessiepatrone in die laboratoriumskaalreaktor
te volg en die moontlike teenwoordigheid en relatiewe hoeveelheid van
organismes, uitsluitende bakterieë, onder sulfaatreduserende kondisies te
bepaal. Analise van T-RFLP en BIOLOGTM Ecoplate het aangedui dat In paar
dominante organismes in die gemeenskap teenwoordig was, wat effens
afgeneem het na verskuiwing na 'n ander koolstofbron. Fluoresserende
hibridisasie het hoër getalle van SRB relatief tot die totale mikrobiese
gemeenskap aangedui as konvensionele kultuur tegnieke. Mikroskopiese
analises het verder getoon dat benewens SRB en ander bakterieë ook giste
en filamentagtige swamme onder sulfaatreduserende kondisies in "n biofilm
geïntegreer was. Hierdie mikroskopiese waarneminge is bevestig deur
fluoresserende in situ hibridisasie (FISH) en gis Lewe / Dood
lewensvatbaarheid peilers.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/53400 |
| Date | 04 1900 |
| Creators | Liss, Jago Milan |
| Contributors | Wolfaardt, G. M., Maree, J. P., Stellenbosch University. Faculty of Science. Dept. of Microbiology. |
| Publisher | Stellenbosch : Stellenbosch University |
| Source Sets | South African National ETD Portal |
| Language | en_ZA |
| Detected Language | English |
| Type | Thesis |
| Format | 85 p. : ill. |
| Rights | Stellenbosch University |
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