This research investigates the ability of rat tapeworm (Hymenolepis diminuta) to accumulate zinc in tissue and the influence of its intake and excretion by its host (laboratory rat). The host was fed by food with zinc in two forms: 1) a mixture of standard food ST1 with hyperaccumulator plant Arabidopsis halleri, 2) ST1 mixture with zinc lactate, which is ordinarily used as a feed supplement for increasing zinc content and it is often included in human diet supplements. Rat control group fed by ST1 only was included in the experiment for verification of the difference. Rats were divided to six groups (OO, OT, RT, RO, MO a MT). Three rat groups were infected by rat tapeworm (OT, RT, MT) and three rat groups were not infected (OO, RO, MO). The control groups OO and OT were fed by ST1 only. RO and RT groups were fed by ST1 with admixture of Arabidopsis halleri and groups MO and MT were fed by ST1 with zinc lactate. Urine and excrements of the rats were collected twice a week during the experiment and their amounts were measured every day. The rats were weighted every week. At the end of the balance phase of the experiment rats were euthanized and seven selected tissues were removed (liver, kidney, spleen, small intestine, testis, muscle and bone). Rat tapeworm was removed from the infected rats. Blood was drawn from the rats. The results show that rat groups infected by the rat tapeworm had lower concentration of zinc in almost all analyzed tissues except for spleen, where the concentration of zinc was the same as in groups without the rat tapeworm. Based on the results the rat tapeworm also has an influence on the excretion of feces and urine.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:259687 |
| Date | January 2016 |
| Creators | Sloup, Vladislav |
| Contributors | Jankovská, Ivana |
| Publisher | Česká zemědělská univerzita v Praze |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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