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Recognizing biological and technical differences in scRNAseq : A comparison of two protocols

Recent advances in sequencing technology have given access to information extracted on a single cell level. Single cell RNA sequencing enables for transcriptomes to be sequenced, allowing for studies within and between cell types. A recently developed protocol, based on Smart-seq2, and the Proximity ligation essay, allows for the detection of protein data from single cells, in parallel with RNA. The combination of the transcriptomic and proteomic data will enhance researchers’ ability to explore cell states. In this study, we are comparing a new pulldown protocol with the widely-used Smart-seq2, as well as against FACS sorted cells. Our results show differences in the RNA sequenced between the two protocols, as well the prediction of cell cycle state based on their data. Using RNA extracted from the pulldown protocol in different time points, we also calculate the direction of development for the cells. We expect that the incorporation of proteomic data will shed light to relevant biological questions related to the cell function.

Identiferoai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-366169
Date January 2018
CreatorsBampalikis, Dimitrios
PublisherUppsala universitet, Institutionen för biologisk grundutbildning
Source SetsDiVA Archive at Upsalla University
LanguageEnglish
Detected LanguageEnglish
TypeStudent thesis, info:eu-repo/semantics/bachelorThesis, text
Formatapplication/pdf
Rightsinfo:eu-repo/semantics/openAccess

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