The 37kDa/67kDa laminin receptor (LRP/LR) is thought to play a major role in the
adhesion to laminin and consequently invasion resulting in the metastasis of tumor
cells. This receptor is reported to be over-expressed in several neoplastic cell lines
and is believed to increase tumor aggressiveness. This research aims at determining
whether the application of anti-LRP/LR specific antibody (IgG1-iS18) on neoplastic
cell lines would result in a decrease in invasion and adhesion. All neoplastic cell lines
had significantly increased cell surface LRP/LR levels compared to NIH/3T3 cells,
with the most notable increase seen in SW480 cells (10.98%). Due to a positive
correlation between the cell surface LRP/LR levels and invasion potential we propose
that an increased LRP/LR level correlates to an increased ability to invade. A
significantly decreased adhesion potential was noted in all neoplastic cell lines except
the non-invasive MCF-7 cell line, upon application of IgG1-iS18, 21% decrease in
HT-1080 cells, 14% in HeLa, 20% in LNCaP, 48% and 74% in A549 and SW480
cells, respectively. Incubation with the anti-LRP/LR antibody IgG1-iS18 resulted in a
significant reduction of the invasive potential of HT-1080 (44%), A549 (33%), HeLa
(69%), SW480 (91%) and LNCaP cells (38%). Furthermore, a high Pearson’s
correlation coefficient between adhesion potential and invasive potential was seen,
confirming that adhesion is indeed a pre-requisite for invasion. The significant
reduction in invasion and adhesion of HT-1080, A549, HeLa, SW480 and LNCaP
cells upon application of the IgG1-iS18 antibody suggests that this macromolecule
might act as a promising therapeutic tool for the treatment of various metastatic
cancer types.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:wits/oai:wiredspace.wits.ac.za:10539/11891 |
| Date | 05 September 2012 |
| Creators | Omar, Aadilah |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | application/pdf |
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