The gastrointestinal microbiota of livestock is a complex and diverse ecosystem, which strongly depends and correlates with animals health. One of the many ways, how to improve the composition of this microbiota in the gastrointestinal tract, is application of probiotics, prebiotics or an appropriate combination called as synbiotics. Using subtle interventions can be achieved health benefits, increase
of some indicators livestock performance and improving the economic situation
of farming.
The aim of the thesis was to find out the effect of prebiotics on the currently applied probiotics and the composition of the intestinal microbiota. Three groups
of calves were studied. In the first one the mixture of five strains of rifampicin resistant variants of Bifidobacterium (RRBif): Bifidobacterium animalis ssp. animalis (2 strains), B. choerinum, B. thermophilum and B. longum ssp. suis was fed to 2 days old calves in a form of fermented milk. In the second group probiotics were adminestered together with prebiotics mixture contains fructooligosaccharides and galactooligosaccharides everyday. The third group without synbiotic treatment was used as a control. The numerous of main bacterial groups in gastrointestinal tract were determined by cultivation.
Our results showed that applied bacteria were able to survive the passage through the gastrointestinal tract of calves. Administered bacteria persist in counts more than 107 CFU/g in the group of calves, that were fed only by probiotics and in numbers 108 CFU/g in the group of calves, that were treated by synbiotics. Furthermore a slow decrease of bifidobacterial numbers to 104 CFU / g was observed in both groups. Significant difference (p = 0.05) between the count of RRBif in probiotic and synbiotic group were determined only two days after administration of that additive. Administration of probiotics and synbiotics to calves increased the count of naturally occurring bifidobacteria, but these differences were not significant.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:257932 |
| Date | January 2016 |
| Creators | Štěrba, Vojtěch |
| Contributors | Vlková, Eva, Jitka, Jitka |
| Publisher | Česká zemědělská univerzita v Praze |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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