This research studied the effects of dietary lipids unsaturation or oxidation on growth, body composition, tissue oxidative status, and health of the juvenile cobia. Five isonitrogenous and isoenergetic experimental diets containing 20% crude lipid, among which 9% was fish oil and 11% was various supplemental oils, were evaluated. The supplemental oils included palm oil (FP), soybean oil (FS), fish oil (FO), oxidized fish oil (OF) and fish oil supplemented with 300 mg/kg diet vitamin E (FE). The degree of lipid oxidation in FE, FO and OF was increased progressively, and so was the degree of unsaturation in FP, FS and FO. The results of a 10-week growth trial showed that lipid unsaturation didn¡¦t affect fish groeth significantly, but lipid oxidation significantly reduced growth. Decrease in lipid unsaturation and increase in lipid oxidation significantly reduced the crude lipid level of ventral muscle but not that of dorsal muscle and liver. Decreasing lipid unsaturation significantly affected serum TC and LDL concentration. The tissue fatty acid composition was significantly affected by the dietary fatty acid composition. Degree of dietary lipid Oxidation was negatively correlated with tissue n-3 PUFA and HUFA concentrations. Muscle TBARS concentrations was significantly affected by dietary lipids oxidation, but not was liver TBARS. Muscle TBARS was decreased as dietary lipid unsaturation was decreased, especially in dorsal muscle. The degree of dietary lipid oxidation of was positively correlated with tissue TBARS. The supplement additional of vitamin E reduced muscle TBARS. Liver catalase activity was decreased when dietary lipid unsaturated was decreased. Even though lysozyme activity in serum, SOD activity in erythrocyte, oxidative burst activity and phagocytic activity in neutrophils, and intracellular superoxide anion ratio in head kidney macrophages were not significantly affected by dietary lipids unsaturation or oxidation, these parameters showed the lowest levels in the group of fish fed the OF diet, indicating the adverse effects of oil oxidation on fish immunity. The results of this research show that oxidation of dietary lipids was more prominent than unsaturation in affecting growth, body lipid composition, peroxidative status, and health of cobia.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0729109-115806 |
| Date | 29 July 2009 |
| Creators | Shiau, Jia-pei |
| Contributors | Chen-Chih Kau, Houng-Yung Chen, Chen-Huei Huang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729109-115806 |
| Rights | not_available, Copyright information available at source archive |
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