Zinc is an essential mineral that has many biological and structural functions in the cells. In Sweden, zinc analysis is performed with different methods and test tubes. The recommended value of zinc is between 11-17 µmol/L together with normal albumin rate. The aim of this study was to validate the colorimetric method for analysis of S-Zinc by comparing the results from two Beckman Coulter AU680 instruments for the insertion of these at the Department of Clinical Chemistry and Transfusion Medicine, Country council of Kalmar. Precision study, correlation study and comparison study was made between the kinds of test tubes BD Vacutainer® Blood Collection Tubes, one consisting of a gel separator, one without contents and the last type contained silica particles. A correlation study was made by comparing patient samples obtained from the department of clinical chemistry, Country council of Jönköping. In the comparison study, serum probes were provided by the staff at the Department of Clinical Chemistry, Kalmar. The blood samples were taken in two kinds of blood collection tubes without contents (one containing silica particles) and one blood collection tubes with gel. The serum tests were analyzed for 4 days and the test results were studied and compared between the collection types. The conclusion of the study was that S-Zink on the Beckman Coulter AU680 instrument with reagents from Sentinel Diagnostic was acceptable. Both Beckman Coulter AU680 instruments provided equivalent results. The correlation between Beckman Coulter AU680 and Thermo Konelab Prime 30i was acceptable. The correlation coefficient showed a high correlation rate between the different methods (r=0, 93). The sustainability of the serum samples, that was poured in new tubes and stored in 2-8°C for 4 days, was good. The study showed distinct differences when analyzing different blood collection tubes, were the collection tube containing gel showed a higher zinc rate. When insertion of the new method, S-Zink, it is recommended that blood collection tubes without contents are used, because of the risk of zinc contamination from collection tubes with gel and plastic stoppers.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:lnu-76650 |
| Date | January 2018 |
| Creators | Shek, Jennifer |
| Publisher | Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB) |
| Source Sets | DiVA Archive at Upsalla University |
| Language | Swedish |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0018 seconds