This research project deals with the characterization of self-replicating Kirsten murine viral DNA. The replication of this viral DNA and tetracycline resistance conferred to bacteria by this viral DNA will be studied. The restriction endonuclease and Southern blot analysis revealed a fragment of pBR322 from the Hind III and Pst I site that is located in the 3' end of the MLV-K:E molecule. Single stranded sequencing of the two terminal ends of this fragment verified that the 3' end of MLV-K:E contains identical sequence homology to pBR322. The presence of this pBR322 fragment explains the unusual properties of the MLV-K:E molecule. However, tetracycline resistance is less in E. Coli containing MLV-K:E than E. coli containing pBR322 as determined by zone of inhibition assay. This may be due to alteration in the promoter region of the tetracycline gene.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc798479 |
| Date | 08 1900 |
| Creators | Najmabadi, Hossein |
| Contributors | O'Donovan, Gerard A., Knesek, John, White, Olivia Masih, Donahue, Manus J., Goven, Arthur James, 1950- |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | xi, 104 leaves : ill., Text |
| Rights | Public, Najmabadi, Hossein, Copyright, Copyright is held by the author, unless otherwise noted. All rights |
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