Voltammetric methods for the determination of diazepam (DZ) and nordiazepam (NDZ) were developed. Techniques differential pulse voltammetry (DPV) and DC voltammetry were used for determination of DZ and NDZ at meniscus modified silver solid amalgam electrode (m-AgSAE). The effect of pHa on the intensity of signal was observed in the mixture of Britton-Robinson buffer and methanol (9:1), and in the mixture of 0.1 mol.l−1 NaOH and methanol (9:1). The stability of the signal during repeated measurements in the mixture of 0.1 mol.l−1 NaOH and methanol (9:1), and in the mixture of BR buffer and methanol (9:1) was monitored. Optimal pHa 13.2 of medium of 0.1 mol.l−1 NaOH and methanol (9:1) was used for determination of DZ with DPV and DCV techniques. Optimal pHa 10.1 of medium of BR buffer and methanol (9:1) was used for determination of NDZ with DPV and DCV techniques. Under these conditions linear dependencies calibration were measured. Concentration range of DZ was measured with DCV in range of 10x10−5 - 6x10−6 mol.l−1 and with DPV technique in range of 10x10−5 - 2x10−6 mol.l−1 . Concentration range of NDZ was measured with DCV technique in range of 10x10−5 - 4x10−6 mol.l−1 and with DPV technique in range of 10x10−5 - 2x10−6 mol.l−1 . The limit of detection for DZ was calculated 6.6x10−6 mol.l−1 with DCV and...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:324640 |
| Date | January 2013 |
| Creators | Samiec, Petr |
| Contributors | Fischer, Jan, Vyskočil, Vlastimil |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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