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Ephippia Production, Activation, and Use of Ex-Ephippio Neonates of Ceriodaphnia Dubia as Toxicity Test Organisms

Ceriodaphnia dubia is widely used as a test organism in monitoring water quality. At the present time, cultures must be continuously maintained in the laboratory. In an attempt to avoid continuous culture and maintenance, the hatching of ephippial eggs of C. dubia would provide test organisms when needed. In order to determine the parameters required for maximum hatching, approximately ninety-four thousand ephippia were exposed to a variety of conditions ranging from light and temperature regimes to drying and freezing. A low hatching yield occurred which is believed to be caused by diminished ephippia viability and/or fertility. To evaluate factors influencing the viability and fertility rate, stains of embryos were examined as were male to female ratios and mating experiments.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc501147
Date08 1900
CreatorsPoage, Duane W.
ContributorsWaller, William T., Dickson, Kenneth L., Atkinson, Samuel F.
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatviii, 80 leaves: ill., Text
RightsPublic, Poage, Duane W., Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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