Return to search

A study of molecular weight (MW) and molecular weight distribution (MWD) of water-insoluble polysaccharides in ganoderma lucidum using MALDI-MS.

Sun Baizhong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 75-79). / Abstracts in English and Chinese. / ABSTRACT --- p.i / ABSTRACT (IN CHINESE) --- p.ii / TABLE OF CONTENTS --- p.iii / LIST OF FIGURES --- p.v / LIST OF TABLES --- p.vii / ABBREVIATIONS --- p.vi / ACKNOWLEDGEMENT --- p.ix / DECLARATION --- p.x / Chapter CHAPTER ONE --- INTRODUCTION / Chapter 1.1 --- Polysaccharides --- p.1 / Chapter 1.2 --- Pharmacological importance of polysaccharides --- p.2 / Chapter 1.3 --- Polysaccharides from Ganoderma Lucidum --- p.3 / Chapter 1.4 --- Characterization of polysaccharides --- p.3 / Chapter 1.5 --- Matrix-assisted laser desorption / ionization --- p.5 / Chapter 1.6 --- Matrix-assisted laser desorption / ionization of polysaccharides --- p.8 / Chapter 1.7 --- Outline of the project --- p.10 / Chapter CHAPTER TWO --- INSTRUMENTATION AND EXPERIMENTAL / Chapter 2.1 --- Time-of-flight mass spectrometry --- p.12 / Chapter 2.1.1 --- Instrumentation --- p.15 / Chapter 2.1.1.1 --- Laser system --- p.16 / Chapter 2.1.1.2 --- Ion source --- p.16 / Chapter 2.1.1.3 --- Reflector --- p.19 / Chapter 2.1.1.4 --- Detector --- p.19 / Chapter 2.1.1.5 --- Data acquisition and manipulation --- p.20 / Chapter 2.2 --- Ultraviolet-visible spectrometer --- p.21 / Chapter CHAPTER THREE --- OPTIMIZATION OF MALDI CONDITIONS FOR POLYSACCHARIDE ANALYSIS USING DMSO AS SOLVENT / Chapter 3.1 --- Introduction --- p.22 / Chapter 3.2 --- Experimental --- p.25 / Chapter 3.2.1 --- Selection of matrix materials --- p.26 / Chapter 3.2.2 --- Selection of co-matrix materials --- p.26 / Chapter 3.2.3 --- Ratios of matrix-to-analyte --- p.26 / Chapter 3.2.4 --- Effect of sample drying temperature --- p.27 / Chapter 3.2.5 --- Sample loading methods --- p.27 / Chapter 3.3 --- Results and discussion --- p.27 / Chapter 3.3.1 --- Selection of matrix materials --- p.28 / Chapter 3.3.2 --- Selection of co-matrix materials --- p.30 / Chapter 3.3.3 --- Effect of matrix-to-co-matrix ratio --- p.32 / Chapter 3.3.4 --- Effect of sample drying temperature --- p.37 / Chapter 3.3.5 --- Effect of matrix-to-analyte ratio --- p.39 / Chapter 3.3.6 --- Evaluation of sample preparation protocols --- p.43 / Chapter 3.4 --- Conclusion --- p.46 / Chapter CHAPTER FOUR --- MOLECULAR AND MOLECULAR WEIGHT DISTRIBUTION OF WATER-INSOLUBLE POLYSACCHARIDES FROM GANODERMA LUCIDUM / Chapter 4.1 --- Introduction --- p.47 / Chapter 4.2 --- Experimental --- p.48 / Chapter 4.2.1 --- Extraction of water-insoluble polysaccharides from Ganoderma Lucidum --- p.48 / Chapter 4.2.2 --- Gel permeation chromatography of water-insoluble polysaccharides --- p.49 / Chapter 4.2.3 --- Phenol sulfuric acid assay --- p.51 / Chapter 4.2.4 --- MALDI-TOF analysis --- p.51 / Chapter 4.2.5 --- Bradford assay --- p.52 / Chapter 4.3 --- Results and discussion --- p.52 / Chapter 4.3.1 --- Extraction of water-insoluble polysaccharides from Ganoderma Lucidum --- p.52 / Chapter 4.3.2 --- Fractionation of water-insoluble polysaccharides using gel permeation chromatography (GPC) --- p.54 / Chapter 4.3.3 --- MALDI-TOF analysis of fractionated polysaccharides --- p.57 / Chapter 4.3.4 --- Carbohydrate content analysis of fractionated polysaccharides --- p.62 / Chapter 4.3.5 --- MW and MWD of water-insoluble polysaccharides extracted from Ganoderma Lucidum --- p.69 / Chapter 4.4 --- Conclusion --- p.71 / Chapter CHAPTER FIVE --- CONCLUDING REMARKS / Chapter 5.1 --- Conclusion --- p.73 / REFERENCES --- p.76 / APPENDIX --- p.81 / Appendix A Reaction scheme of carbohydrate with phenol in phenol-sulfuric acid assay --- p.81

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324686
Date January 2004
ContributorsSun, Baizhong., Chinese University of Hong Kong Graduate School. Division of Chemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, x, 80 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Page generated in 0.0021 seconds