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Dna electrophoresis in photopolymerized polyacrylamide gels on a microfluidic device

DNA gel electrophoresis is a critical analytical step in a wide spectrum of genomic
analysis assays. Great efforts have been directed to the development of
miniaturized microfluidic systems (“lab-on-a-chip” systems) to perform low-cost,
high-throughput DNA gel electrophoresis. However, further progress toward
dramatic improvements of separation performance over ultra-short distances requires
a much more detailed understanding of the physics of DNA migration in
the sieving gel matrix than is currently available in literature. The ultimate goal
would be the ability to quantitatively determine the achievable level of separation
performance by direct measurements of fundamental parameters (mobility, diffusion,
and dispersion coefficients) associated with the gel matrix instead of the
traditional trial-and-error process.
We successfully established this predicting capability by measuring these fundamental
parameters on a conventional slab gel DNA sequencer. However, it is difficult to carry out fast and extensive measurements of these parameters on a conventional
gel electrophoresis system using single-point detection (2,000 hours on
the slab gel DNA sequencer we used).
To address this issue, we designed and built a new automated whole-gel scanning
detection system for a systematic investigation of these governing parameters on
a microfluidic gel electrophoresis device with integrated on-chip electrodes, heaters,
and temperature sensors. With this system, we can observe the progress of
DNA separation along the whole microchannel with high temporal and spatial
accuracy in nearly real time. This is in contrast to both conventional slab gel imaging
where the entire gel can be monitored, but only at one time frame after
completion of the separation, and capillary electrophoresis systems that allows
detection as a function of time, but only at a single detection location.
With this system, a complete set of mobility, diffusion, and dispersion data can be
collected within one hour instead of days or even months of work on a conventional
sequencer under the same experimental conditions. The ability to acquire
both spatial and temporal data simultaneously provides a more detailed picture of
the separation process that can potentially be used to refine theoretical models
and improve separation performance over ultra-short distances for the nextgeneration
of electrophoresis technology.

Identiferoai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-2685
Date15 May 2009
CreatorsLo, Chih-Cheng
ContributorsUgaz, Victor M.
Source SetsTexas A and M University
Languageen_US
Detected LanguageEnglish
TypeBook, Thesis, Electronic Dissertation, text
Formatelectronic, application/pdf, born digital

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