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Antiviral activity of the medicinal plants, Adina pilulifera, Narcissus tazetta and Wikstroemia indica, against respiratory syncytial virus.

Ho, Wing Shan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 116-137). / Abstracts in English and Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / Abstract (Chinese Version) --- p.v / Table of Contents --- p.vii / List of Figures --- p.x / List of Tables --- p.xi / List of Abbreviations --- p.xii / Chapter Chapter One: --- General Introduction / Chapter 1.1 --- Respiratory Syncytial Virus (RSV) --- p.1 / Chapter 1.2 --- RSV biology --- p.2 / Chapter 1.3 --- RSV strains --- p.10 / Chapter 1.4 --- RSV pathogenesis and host antiviral responses --- p.11 / Chapter 1.5 --- Prevention of RSV infection --- p.13 / Chapter 1.5.1 --- Vaccines --- p.13 / Chapter 1.5.2 --- Passive anti-RSV antibodies --- p.17 / Chapter 1.6 --- Treatment for RSV infections --- p.20 / Chapter 1.6.1 --- Ribavirin (Virasole®) --- p.20 / Chapter 1.6.2 --- Other antiviral strategies --- p.21 / Chapter 1.6.2.1 --- Attachment inhibitors --- p.22 / Chapter 1.6.2.2 --- Fusion inhibitors --- p.23 / Chapter 1.6.2.3 --- Replication inhibitors --- p.25 / Chapter 1.6.2.4 --- Ethnobotanic medicines --- p.28 / Chapter 1.6.2.4.1 --- Anti-RSV medicinal plant components --- p.31 / Chapter 1.6.2.4.1.1 --- Phenolics and polyphenols --- p.31 / Chapter 1.6.2.4.1.2 --- Flavonoids --- p.32 / Chapter 1.6.2.4.1.3 --- Terpenoids and essential oils --- p.34 / Chapter 1.6.2.4.1.4 --- Lectins --- p.34 / Chapter 1.6.2.4.1.4.1 --- General introduction to lectins --- p.34 / Chapter 1.6.2.4.1.4.2 --- Historical aspects of lectins --- p.35 / Chapter 1.6.2.4.1.4.3 --- Applications of lectins --- p.36 / Chapter 1.7 --- Objectives of the project --- p.37 / Chapter Chapter Two: --- Screening of medicinal plants and phytochemicals for antiviral activity against RSV / Chapter 2.1 --- Introduction --- p.39 / Chapter 2.2 --- Materials and methods --- p.47 / Chapter 2.2.1 --- Medicinal plants and phytochemicals --- p.47 / Chapter 2.2.2 --- Plant extracts preparation --- p.48 / Chapter 2.2.2.1 --- Aqueous extracts --- p.48 / Chapter 2.2.2.2 --- Ethanol extracts --- p.48 / Chapter 2.2.3 --- Cell and virus --- p.49 / Chapter 2.2.4 --- Endpoint titration of RSV infectivity --- p.50 / Chapter 2.2.5 --- Cytotoxicity test --- p.50 / Chapter 2.2.6 --- Antiviral assay --- p.52 / Chapter 2.3 --- Results --- p.53 / Chapter 2.4 --- Discussion --- p.58 / Chapter Chapter Three: --- "Mechanistic studies of anti-RSV actions of various fractions of Adina pilulifera, and daphnoretin, a purified compound from Wikstroemia indica" / Chapter 3.1 --- Introduction --- p.60 / Chapter 3.2 --- Materials and methods --- p.65 / Chapter 3.2.1 --- Fractionation of A. pilulifera ethanol extract --- p.65 / Chapter 3.2.2 --- Cell and virus --- p.65 / Chapter 3.2.3 --- Cytotoxicity test --- p.65 / Chapter 3.2.4 --- Endpoint titration of RSV by TCID50 method --- p.66 / Chapter 3.2.5 --- Antiviral study by CPE reduction assay --- p.66 / Chapter 3.2.6 --- Endpoint titration of RSV by plaque assay --- p.66 / Chapter 3.2.7 --- Antiviral study by plaque reduction assay --- p.67 / Chapter 3.2.8 --- Mode of antiviral action study --- p.68 / Chapter 3.3 --- Results --- p.70 / Chapter 3.4 --- Discussion --- p.76 / Chapter Chapter Four: --- Antiviral activity of Narcissus tazetta proteins / Chapter 4.1 --- Introduction --- p.81 / Chapter 4.2 --- Materials and methods --- p.88 / Chapter 4.2.1 --- Crude proteins extraction from Narcissus tazetta cultivar --- p.88 / Chapter 4.2.2 --- Separation of proteins with affinity column --- p.88 / Chapter 4.2.3 --- Gel filtration of protein fractions on Superose column --- p.89 / Chapter 4.2.4 --- Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) --- p.89 / Chapter 4.2.5 --- Electroblotting and N-terminal amino acid sequence analysis --- p.90 / Chapter 4.2.6 --- Protein concentration determination --- p.90 / Chapter 4.2.7 --- Isolation and purification of N. tazetta lectin (NTL) --- p.91 / Chapter 4.2.8 --- Antiviral activities of N. tazetta proteins and NTL --- p.92 / Chapter 4.2.8.1 --- Cell and virus --- p.92 / Chapter 4.2.8.2 --- Cytotoxicity test --- p.92 / Chapter 4.2.8.3 --- Endpoint titration of RSV by TCID50 method --- p.92 / Chapter 4.2.8.4 --- Antiviral study by CPE reduction assay --- p.92 / Chapter 4.2.8.5 --- Endpoint titration of RSV by plaque assay --- p.92 / Chapter 4.2.8.6 --- Antiviral study by plaque reduction assay --- p.93 / Chapter 4.2.8.7 --- Mode of antiviral action study --- p.93 / Chapter 4.3 --- Results --- p.94 / Chapter 4.4 --- Discussion --- p.107 / Chapter Chapter Five: --- General Discussion and Conclusions --- p.111 / References --- p.116

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326488
Date January 2008
ContributorsHo, Wing Shan., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiv, 137 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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