In this study, the effectiveness of vaccination against Yersinia ruckeri, using an autogenous bacterin, in rainbow trout (Oncorhynchus mykiss) was assessed. In addition, the immune response detected by enzyme-linked immunosorbent assay (ELISA) was evaluated. Rainbow trout were vaccinated at 25 grams mean body weight by standard bath immersion and intraperitoneal (i.p.) injection (saline-based injection or oil-based adjuvant injection). Bath-challenges occurred on days 42, 101, 181, 286, and 381 post-vaccination. Plasma samples were collected pre-challenge and fourteen days post-challenge for antibody titer analysis. Antibody kinetics were followed prior to first challenge. Relative percent survival (RPS) ranged from 98.96% to 96.85% and did not statistically differ among vaccinated groups. Increased antibody titer was identified in the oil-based adjuvant injection vaccinated group however, statistical significance among vaccinated groups was not determined.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-11182002-180423 |
| Date | 19 November 2002 |
| Creators | Jenkins, Thomas Burton II |
| Contributors | Dr. David A. Dickey, Dr. Michael G. Levy, Dr. Jeffrey M. Hinshaw |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-11182002-180423/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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