The study was focused on study of diversity of Cryptosporidium spp. in wild canines and bears in the Czech Republic, Slovak Republic, Poland and Romania. A total of 359 faecal samples were collected from 179 red foxes (Vulpes vulpes), 83 grey wolves (Canis lupis), 63 brown bears (Ursus arctos) and 34 jackals (Canis aureus). Faecal samples were screened for Cryptosporidium by microscopy and PCR/sequencing. Phylogenetic analysis of small-subunit rRNA, actin and 60-kDa glycoprotein sequences revealed the presence of C. tyzzeri, C. andersoni in red foxes, C. canis and C. ubiquitum in gray wolves and C. galli in a brown bear and a red fox. Subtyping of C. ubiquitum and C. tyzzeri isolate by sequence analysis of the 60-kDa glycoprotein gene showed that isolates belonged to the XIId and IXa subtype family, respectively. Detection of host-non-specific cryptosporidia, except C. canic and C. ubiquitum, in wild canine and bears shows rather a food preference of screened carnivors than on an active infection.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:375817 |
| Date | January 2018 |
| Creators | KELLNEROVÁ, Klára |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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