M.S. / Biochemistry / The white-rot basidiomycete Phanerochaete chrysosporium produces intracellular soluble and particulate ß-glucosidases and an extracellular ß-glucosidase. The extracellular enzyme is induced by cellulose but repressed in the presence of glucose. The molecular weight of this enzyme is 90,000. The K [subscript m] for -p-nitrophenyl-ß-glucoside is 1.6 x 10[superscript -4] M; the K [subscript 1] for glucose, a competitive inhibitor, is 5.0 x 10[superscript -4] M. The K [subscript m] for cellobiose is 5.3 x 10[superscript -4] M. The intracellular soluble enzyme is induced by cellobiose; this induction is prevented by cycloheximide. The presence of 300 mM glucose in the medium, however, had no effect on induction. The K [subscript m] for p-nitrophenyl- ß -glucoside is 1.1 x 10[superscript -4] M. The molecular weight of this enzyme is about 410,000. Both enzymes have an optimal temperature of 60°C and an E [subscript act] of 9.15 kcal/mole. The pH optima, however, were 7.0 and 5.5 for the intracellular and extracellular enzymes, respectively.
| Identifer | oai:union.ndltd.org:OREGON/oai:content.ohsu.edu:etd/114 |
| Date | 04 1900 |
| Creators | Smith, Mark H. |
| Publisher | Oregon Health & Science University |
| Source Sets | Oregon Health and Science Univ. Library |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | Needs Adobe Acrobat Reader to view., pdf, 1067.89 KB |
| Rights | http://www.ohsu.edu/library/etd_rights.shtml |
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