This thesis deals with the monitoring of fungal organisms involved in the decay of bases of common ash trees (Fraxinus excelsior) affected by the ash dieback (Hymenoscyphus fraxineus) infection. Monitoring was carried out in winter 2018 on 50 trees. Samples were taken from tree bases and sorted into 5 health categories. The total area of samples and the area affected by rot was measured in a laboratory. Fungal organisms were isolated from infected and healthy wood and identified by DNA extraction and sequencing. Finally, the determined species were statistically evaluated by a combination of constrained and unconstrained ordination methods. In total 436 pure cultures of fungal organisms were isolated. 23 species of fungal organisms were determined, of which 21 were in symptomatic wood (with a visible rot) and 5 in asymptomatic (healthy) wood. Xylaria polymorpha was confirmed to be present in most trees, it was found in 27 samples - 16× in asymptomatic wood and 11× in symptomatic wood. Hymenoscyphus fraxineus was isolated once from the 1st health category and once from the 5th health category from symptomatic wood.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:428557 |
| Date | January 2019 |
| Creators | Slezáková, Hana |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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