Tse, Chung Kwan Anna. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 86-105). / Abstracts in English and Chinese. / Abstract in English --- p.i / Abstract in Chinese --- p.iii / Acknowledgement --- p.v / Table of content --- p.vii / List of figures and tables --- p.xi / Symbols and abbreviation --- p.xiii / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- Folliculogenesis --- p.1 / Chapter 1.1.1 --- In Mammals --- p.1 / Chapter 1.1.2 --- In Teleosts --- p.2 / Chapter 1.1.2.1 --- Vitellogenesis --- p.3 / Chapter 1.1.2.2 --- Oocyte Maturation --- p.4 / Chapter 1.1.3 --- Oocyte Control of Folliculogenesis --- p.5 / Chapter 1.2 --- Epidermal Growth Factor Family --- p.7 / Chapter 1.2.1 --- Epidermal Growth Factor Ligand Family --- p.7 / Chapter 1.2.1.1 --- Discovery --- p.7 / Chapter 1.2.1.2 --- Structure --- p.8 / Chapter 1.2.1.3 --- Shedding --- p.8 / Chapter 1.2.1.4 --- Functions --- p.9 / Chapter 1.2.2 --- Epidermal Growth Factor Receptor Family --- p.10 / Chapter 1.2.2.1 --- Structure --- p.10 / Chapter 1.2.2.2 --- Ligand Binding and Dimerization --- p.11 / Chapter 1.2.2.3 --- Signaling and Internalization --- p.12 / Chapter 1.2.3 --- EGF Family in Reproduction --- p.13 / Chapter 1.2.3.1 --- Reproductive Function of EGF Family --- p.13 / Chapter 1.2.3.2 --- EGF Network as Downstream Mediator of Gonadotropins --- p.15 / Chapter 1.3 --- Objectives of the Present Study --- p.18 / Chapter Chapter 2 --- Spatiotemporal Expression of the EGF Family in Zebrafish Ovary / Chapter 2.1 --- Introduction --- p.20 / Chapter 2.2 --- Materials and Methods --- p.22 / Chapter 2.2.1 --- Chemicals --- p.22 / Chapter 2.2.2 --- Animals --- p.22 / Chapter 2.2.3 --- Isolation of Different Tissues and Ovarian Follicles --- p.22 / Chapter 2.2.4 --- Separation of Oocyte and Follicle layers --- p.23 / Chapter 2.2.5 --- Embryo Collection --- p.23 / Chapter 2.2.6 --- Tyrphostin AG 1478 Treatment of Embryos --- p.23 / Chapter 2.2.7 --- Total RNA Isolation and Reverse Transcription --- p.24 / Chapter 2.2.8 --- Semi-quantitative and Real-time Polymerase Chain Reaction (PCR) --- p.24 / Chapter 2.2.9 --- Data Analysis --- p.25 / Chapter 2.3 --- Results --- p.27 / Chapter 2.3.1 --- Validation of Semi-quantitative PCR Quantification --- p.27 / Chapter 2.3.2 --- Tissue Distribution of the EGF Family --- p.27 / Chapter 2.3.3 --- Spatial Expression of the EGF and the Activin Families within the Follicles --- p.27 / Chapter 2.3.4 --- Temporal Expression of the EGF Family in Folliculogenesis --- p.29 / Chapter 2.3.5 --- Temporal Expression of the EGF Family in Embryogenesis --- p.29 / Chapter 2.3.6 --- Effect of Blocking EGFR on Embryonic Development --- p.30 / Chapter 2.4 --- Discussion --- p.31 / Chapter Chapter 3 --- Regulation of the EGF Family in Cultured Follicle Cells and Their Effects on the Expression of Activin Subunits / Chapter 3.1 --- Introduction --- p.55 / Chapter 3.2 --- Materials and Methods --- p.58 / Chapter 3.2.1 --- Chemicals --- p.58 / Chapter 3.2.2 --- Primary Follicle Culture --- p.58 / Chapter 3.2.3 --- Ovarian Fragment Incubation --- p.58 / Chapter 3.2.4 --- Total RNA Isolation and Reverse Transcription --- p.58 / Chapter 3.2.5 --- Semi-quantitative and Real-time Polymerase Chain Reaction (PCR) --- p.59 / Chapter 3.2.6 --- Microinjection --- p.59 / Chapter 3.2.7 --- Data analysis --- p.59 / Chapter 3.3 --- Results / Chapter 3.3.1 --- Expression of the EGF Family in Cultured Follicle Cells --- p.61 / Chapter 3.3.2 --- Effects of EGF on the EGF Family Expression in Cultured Follicle Cells --- p.61 / Chapter 3.3.3 --- Effects of BTC and HB-EGF on the Expression of EGF Ligands in Cultured Follicle Cells --- p.61 / Chapter 3.3.4 --- Effect of EGF Ligands on the Expression of Activin Subunits in Cultured Follicle Cells --- p.62 / Chapter 3.3.5 --- Effects of Actinomycin D on Ovarian Fragments --- p.62 / Chapter 3.3.6 --- Effects of Microinjecting Anti-EGF Morpholino on Full Grown Follicles --- p.62 / Chapter 3.4 --- Discussion --- p.64 / Chapter Chapter 4 --- General Discussion --- p.81 / References --- p.86
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326491 |
| Date | January 2008 |
| Contributors | Tse, Chung Kwan Anna., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xv, 105 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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