Eight Xenopus laevis claudin genes, Xcla1, Xcla4B, Xcla5, Xcla6, Xcla12, Xcla16, Xcla18 and Xcla19, were cloned and sequenced. Their normal mRNA expression was determined from cleavage stage to tadpole stage by whole mount in situ hybridization. The protein expression of Xcla5 was detected at the neural stage by whole mount immunostaining. Overexpression of Xcla5 by injection of synthetic mRNA into embryos caused morphological defects similar to those in embryos exposed to Bisphenol A (BPA). Altered patterns of claudin gene expression in the presence of BPA can be correlated with these developmental defects. The results suggest that claudins may play an important role in neural crest cell migration, epithelial-mesenchymal transition and ultimately organogenesis during embryonic development.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-04272005-222011 |
| Date | 28 April 2005 |
| Creators | XIE, JIANZHEN |
| Contributors | Robert Grossfeld, Brenda Brizuela, Pat Estes |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-04272005-222011/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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